The presented bachelor thesis deals with the adhesion of materials for horizontal road marking in the case of renewal of marking and two-phase marking. Within the work specified parameters were measured and evaluated. The theoretical part describes the initial situation and is followed by the general characteristic of the horizontal road marking. Further, there are also described individual tests performed on horizontal road marking carried out within the presented bachelor thesis. The practical part deals with the preparation, production and description of the samples that have been used for this work. The evaluation of the measurements carried out on all testing samples of the marking follows. All evaluations are based on test results and photo documentation.