Course detail

Cell and Tissue Engineering

FEKT-MPA-BTIAcad. year: 2026/2027

This course provides a comprehensive overview of the principles and methodologies of cellular and tissue engineering with a focus on applications in regenerative medicine. It covers the fundamentals of biomaterials used in tissue engineering, scaffold design and fabrication, cell culture techniques, and the use of bioreactors. The course further addresses the role of stem cells, mechanotransduction processes, and strategies for engineering vascular, skin, bone, and cartilage tissues, as well as organ-on-chip systems. Emphasis is placed on cell–biomaterial interactions, advanced characterization techniques for biomaterials and cells, and the clinical translation of engineered tissues, including regulatory and ethical considerations.

Language of instruction

English

Number of ECTS credits

4

Mode of study

Not applicable.

Entry knowledge

Basic knowledge of biology.

Rules for evaluation and completion of the course

Lecture – final test

Practical sessions – 100% attendance required

Aims

1 – Cell and Tissue Engineering Fundamentals

2 – Biomaterials in Tissue Engineering

3 – Scaffold Design & Fabrication for Tissue Engineering

4 – Cell Culture Techniques & Bioreactors

5 – Stem Cells in Regenerative Medicine

6 – Mechanotransduction in Cell Engineering

7 – Blood Vessel and Skin Tissue Engineering

8 – Bone and Cartilage Tissue Engineering

9 – Organ–on–a–Chip Systems

10 – Cell-biomaterial Interaction and Characterization

11 – Characterization Techniques of Biomaterials and Cells

12 – Clinical Translation & Regulatory Aspects

Study aids

1. Haraguchi Y, Shimizu T, Yamato M, Okano T. Concise review: cell therapy and tissue engineering for cardiovascular disease. Stem Cells Transl Med. 2012 Feb;1(2):136-41. Epub 2012 Jan 26. PMID: 23197760; PMCID: PMC3659688.

2. Cheng, B., Li, M., Lin, M. et al. Mechanobiology across timescales. Nat Rev Phys 7, 621–644 (2025).

3. Shiva Norouzi, Nikoo Saveh Shemshaki, Ehsan Norouzi, Masoud Latifi, Bahareh Azimi, Serena Danti, Xiaolan Qiao, Yuee Miao, Shengyuan Yang, Mohsen Gorji, Vasilije Petrovic, M. Ali Aboudzadeh, Roohollah Bagherzadeh, Recent advances in biomaterials for tissue-engineered constructs: Essential factors and engineering techniques, Materials Today Chemistry, Volume 37, 2024, 102016, ISSN 2468-5194,

4. Agarwal T, Onesto V, Banerjee D, Guo S, Polini A, Vogt C, Viswanath A, Esworthy T, Cui H, O'Donnell A, Vajanthri KY, Moroni L, Ozbolat IT, Panoskaltsis-Mortari A, Zhang LG, Costantini M, Maiti TK. 3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation. Biofabrication. 2025 Aug 7;17(4).  PMID: 40513614.

Prerequisites and corequisites

Not applicable.

Basic literature

Haraguchi Y, Shimizu T, Yamato M, Okano T. Concise review: cell therapy and tissue engineering for cardiovascular disease. Stem Cells Transl Med. 2012 Feb;1(2):136-41. doi: 10.5966/sctm.2012-0030. Epub 2012 Jan 26. PMID: 23197760; PMCID: PMC3659688. (EN)

Recommended reading

Cheng, B., Li, M., Lin, M. et al. Mechanobiology across timescales. Nat Rev Phys 7, 621–644, (2025). (EN)
Agarwal T, Onesto V, Banerjee D, Guo S, Polini A, Vogt C, Viswanath A, Esworthy T, Cui H, O'Donnell A, Vajanthri KY, Moroni L, Ozbolat IT, Panoskaltsis-Mortari A, Zhang LG, Costantini M, Maiti TK. 3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation. Biofabrication. 2025 Aug 7;17(4). doi: 10.1088/1758-5090/ade47a. PMID: 40513614.
Shiva Norouzi, Nikoo Saveh Shemshaki, Ehsan Norouzi, Masoud Latifi, Bahareh Azimi, Serena Danti, Xiaolan Qiao, Yuee Miao, Shengyuan Yang, Mohsen Gorji, Vasilije Petrovic, M. Ali Aboudzadeh, Roohollah Bagherzadeh,Recent advances in biomaterials for tissue-engineered constructs: Essential factors and engineering techniques, Materials Today Chemistry, Volume 37, 2024, 102016, ISSN 2468-5194, (EN)

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

Syllabus

1 – Cell and Tissue Engineering Fundamentals

2 – Biomaterials in Tissue Engineering

3 – Scaffold Design & Fabrication for Tissue Engineering

4 – Cell Culture Techniques & Bioreactors

5 – Stem Cells in Regenerative Medicine

6 – Mechanotransduction in Cell Engineering

7 – Blood Vessel and Skin Tissue Engineering

8 – Bone and Cartilage Tissue Engineering

9 – Organ–on–a–Chip Systems

10 – Cell-biomaterial Interaction and Characterization

11 – Characterization Techniques of Biomaterials and Cells

12 – Clinical Translation & Regulatory Aspects

Laboratory exercise

9 hours, compulsory

Teacher / Lecturer

Syllabus

1. Cultivation of cells. Preparation of hydrogel and seeding of cells on hydrogel. Degradation of hydrogel.

2. Swelling of hydrogel. Evaluation of hydrogel swelling and degradation. Microscopic evaluation of cells on hydrogel (VIS, fluorescence).

3. Basic of Fusion 360. Virtual reality for Fusion model. 3D printing and segmentation (Slicer) in cardiology + practical 3D printing.

Individual preparation for laboratories

40 hours, compulsory

Teacher / Lecturer

Syllabus

Odborná příprava k tématu a nastudovaní materiálů k laboratorním cvičením.

Individual preparation for a final exam

25 hours, compulsory

Teacher / Lecturer