Course detail
Advanced Light Microscopy - Imaging Theory
FSI-9MIAAcad. year: 2024/2025
The course explains principles of light microscopy of three-dimensional objects in terms of wave optics. Image properties are described using the formalism of 3D pupil and transfer functions. Properties of advanced microscopic techniques for 3D imaging are explained, especially those of confocal microscopy and coherence-controlled holographic microscopy.
Language of instruction
Czech
Mode of study
Not applicable.
Guarantor
Department
Entry knowledge
Wave and Fourier optics, geometrical optics, fundamentals of light microscopy.
Rules for evaluation and completion of the course
Examination is oral. Detailed knowledge of explained matter is proved. Examined student can use a literature.
Presence at lectures is not obligatory, but recommended.
Presence at lectures is not obligatory, but recommended.
Aims
Understanding of principles of 3D light microscopy and microscopic imaging.
Acquiring of methods for fundamental 3D imaging characteristics calculation.
Knowledge of fundamentals of theoretical description of microscope imaging of 3D objects of their relation to experiment and competence to apply them in practice.
Acquiring of methods for fundamental 3D imaging characteristics calculation.
Knowledge of fundamentals of theoretical description of microscope imaging of 3D objects of their relation to experiment and competence to apply them in practice.
Study aids
Not applicable.
Prerequisites and corequisites
Not applicable.
Basic literature
Min Gu: Principles of Three-Dimensional Imaging in Confocal Microscopes (EN)
Radim Chmelik et al.: The Role of Coherence in Image Formation in Holographic Microscopy, Progress in Optics 59 (2014) 267-335. (EN)
Radim Chmelík.: Trojrozměrné zobrazení v mikroskopii. FSI VUT v Brně, Brno 2003 (CS)
Tony Wilson: Confocal Microscopy. Academic Press, London 1990 (EN)
Radim Chmelik et al.: The Role of Coherence in Image Formation in Holographic Microscopy, Progress in Optics 59 (2014) 267-335. (EN)
Radim Chmelík.: Trojrozměrné zobrazení v mikroskopii. FSI VUT v Brně, Brno 2003 (CS)
Tony Wilson: Confocal Microscopy. Academic Press, London 1990 (EN)
Recommended reading
J. W. Goodman: Introduction to Fourier Optics. 2nd ed.. McGraw-Hill, New York 1996 (EN)
M. Born, E. Wolf: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999 (EN)
M. Born, E. Wolf: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999 (EN)
Classification of course in study plans
Type of course unit
Lecture
20 hod., optionally
Teacher / Lecturer
Syllabus
1. Important knowledge in the field of light microscopy and wave optics
2. Light wave interaction with an object
3. Imaging from the point of view of wave optics
4. Imaging by confocal micorscope
5. Imaging by interference and holographic microscope
6. Imaging by fluorescence microscope
7. Imaging in bright field
8. Another specific cases of imaging
2. Light wave interaction with an object
3. Imaging from the point of view of wave optics
4. Imaging by confocal micorscope
5. Imaging by interference and holographic microscope
6. Imaging by fluorescence microscope
7. Imaging in bright field
8. Another specific cases of imaging