Course detail

Optical Microscopy and Spectroscopy

FSI-TMKAcad. year: 2024/2025

Introduction to light microscopy (historical overview completed by substantial pieces of knowledge of geometric and wave optics, light-microscope optical setup, techniques of light microscopy, and practical knowledge), theoretical description of image formation (wave theory of image formation based on the Abbe theory), fluorescence microscopy, confocal microscopy, interference and holographic microscopy (principle, setup of the device, imaging properties), X-ray computed tomography, spectroscopic methods (Laser-Induced Breakdown, UV, VIS, Raman), polarisation microscopy, computers and cameras in microscopy.


Demonstrations and practical exercises on light microscopy, and spectroscopy and particle spectroscopy are carried out in laboratories.


Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

doc. Ing. Daniel Zicha, CSc.

Department

Institute of Physical Engineering (ÚFI)

Entry knowledge

Elementary Physics, Quantum Physics, Solid State Physics, Surfaces and Thin Films, Geometrical and wave optics.

Rules for evaluation and completion of the course

The assessment of a student is made upon his performance in written and oral part of the exam.

The presence of students is monitored by the tutor. Maximum of tolerated absence is 25 %.

Aims

The goal of the course is to provide students an overview of the principles and methods of light microscopy and spectroscopy, and give them practical experience with relevant devices.
Students will learn about the history and modern techniques and approaches in the field of light microscopy and spectroscopy and have practical experience with relevant devices.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

H. Friedrich: Scattering Theory. Springer, Heidelberg, New York, Dordrecht, London 2013. (EN)
A. R. Hibbs: Confocal Microscopy for Biologists. Springer, 2004.
D. B. Murphy, M.W. Davidson: Fundamentals of light microscopy and electronic imaging. Wiley‐Blackwell 2012. (EN)
E. Keprt: Teorie optických přístrojů 2, Teorie a konstrukce mikroskopu, SPN, Praha 1966. (CS)
H. Kuzmany: Solid-state spectroscopy. Springer, 2009. (EN)
Internetové zdroje: http://micro.magnet.fsu.edu/primer http://microscopyu.com (EN)
R. Chmelík: Materiály do praktika předmětu Mikroskopie a spektroskopie. Elektronický studijní text, Brno, 2014. (CS)

Recommended reading

Not applicable.

Elearning

eLearning: currently opened course

Classification of course in study plans

  • Programme N-FIN-P Master's 2 year of study, winter semester, compulsory
  • Programme N-PMO-P Master's 2 year of study, winter semester, compulsory

  • Programme C-AKR-P Lifelong learning

    specialization CZS , 1 year of study, winter semester, elective

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

doc. Ing. Tomáš Zikmund, Ph.D.
doc. Ing. Daniel Zicha, CSc.
prof. RNDr. Josef Humlíček, CSc.
doc. Ing. Pavel Pořízka, Ph.D.
prof. RNDr. Radim Chmelík, Ph.D.
Ing. Filip Ligmajer, Ph.D.
Mgr. Ota Samek, Ph.D.
prof. RNDr. Michal Kozubek, Ph.D.
Ing. Hana Uhlířová, Ph.D.
Ing. Zbyněk Dostál, Ph.D.

Syllabus

Introduction to light microscopy
Theory of imaging in microscopy
Fluorescence microscopy
Confocal and 2-photon microscopy
Interference and holographic microscopy
4G in holographic interferometry
X-ray computed tomography
Interaction of light with matter
Laser-induced breakdown spectroscopy
Optical and Raman spectroscopy
Computers and cameras in microscopy
Polarization microscopy

Laboratory exercise

13 hod., compulsory

Teacher / Lecturer

Ing. Zbyněk Dostál, Ph.D.
prof. RNDr. Radim Chmelík, Ph.D.
doc. Ing. Pavel Pořízka, Ph.D.
prof. RNDr. Josef Humlíček, CSc.
Ing. Filip Ligmajer, Ph.D.
Ing. Hana Uhlířová, Ph.D.
Mgr. Ota Samek, Ph.D.
doc. Ing. Tomáš Zikmund, Ph.D.
prof. RNDr. Michal Kozubek, Ph.D.
doc. Ing. Daniel Zicha, CSc.

Syllabus

Demonstrations and practical exercises in laboratories follow the lectures.

Elearning

eLearning: currently opened course