Course detail

Technology in Cellular Engineering

FEKT-MPA-TBIAcad. year: 2023/2024

Not applicable.

Language of instruction

English

Number of ECTS credits

5

Mode of study

Not applicable.

Guarantor

Ing. Vratislav Čmiel, Ph.D.

Department

Department of Biomedical Engineering (UBMI)

Offered to foreign students

The home faculty only

Entry knowledge

Knowledge of biology, mathematics and physics accordant with the subjects of previous bachelor’s study is expected.

Rules for evaluation and completion of the course

The final grade for the course will be determined by the scores obtained from the midterm tests and the activity
exercises (max. 30 points in total) and the final written examination (max. 70 points).
Only students who have been awarded credit may take the final exam.
- obtaining at least 15 points in the laboratory exercises, including points for the continuous written tests,
- a non-zero score on the continuous written tests,
- a non-zero score on the protocols or homework assignments,
- meeting full attendance requirements.

More detailed conditions for successful completion of the course are set out in the annually updated decree of the course coordinator.  

 

Aims

Not applicable.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Alberts B., Johnson A., Walter P.: Molecular Biology of the Cell. (CS)
José Luis R. Arrondo, Alicia Alonso: Advanced Techniques in Biophysics. (CS)
Bengt Nölting: Methods in Modern Biophysics (CS)
Ankur Singh, Akhilesh K. Gaharwar: Microscale Technologies for Cell Engineering (CS)

Recommended reading

Not applicable.

eLearning

eLearning: currently opened course

Classification of course in study plans

  • Programme MPC-BTB Master's, any year of study, summer semester, elective
  • Programme MPC-BIO Master's, 2. year of study, summer semester, compulsory
  • Programme MPAD-BIO Master's, 2. year of study, summer semester, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

Mgr. Bc. Darina Čejková, Ph.D.
Ing. Vratislav Čmiel, Ph.D.
Amir Hashemi
Larisa Chmelíková, Ph.D.
Dr. Rima Paul

Syllabus

1. Cultivation and preparation of cells and tissue, automation of processes in the cell laboratory.
2. Technology for cell counting and sorting, automated laboratory analyzers for tissue engineering.
3. Microscopic techniques, bright field observation, contrast techniques, fluorescence microscopy.
4. Special microscopic imaging techniques, DHM, AFM.
5. Electron microscopy in cell engineering, correlation microscopy, preparation of biological samples for electron microscopy.
6. Application of biomaterials in biomedical engineering.
7. 3D bioprinting and its application in regenerative medicine.
8. Nanotechnology in regenerative and cancer medicine.
9. Mechanical properties of cells and methods of their measurement.
10. Methods and technologies for cell migration analysis.
11. Technologies and methods used in optogenetics.
12. Technologies applied to animal models. Virtual reality in biomedical applications.  

Exercise in computer lab

13 hours, compulsory

Teacher / Lecturer

Ing. Vratislav Čmiel, Ph.D.
Masoumeh Ezati
Amir Hashemi
Larisa Chmelíková, Ph.D.
Minoo Partovi Nasr
Ing. Inna Zumberg

Syllabus

1. Cell engineering - cultivation, observation
2. TEER measurement of cultivated cells
3. Observation techniques, fluroescence microscopy
4. Cells segmentation (image analysis)
5. Cells migrations, data processing
6. Signals processing MEA+AFM
7. Fusion 360 basics
8. 3D printing in cardiology, segmentation (Slicer)
9. Biomaterials preparation
10. 3D bioprinting: practical task (Inkredible)
11. Virtual Reality for Fusion models
12. Excursion  

Laboratory exercise

13 hours, compulsory

Teacher / Lecturer

Ing. Vratislav Čmiel, Ph.D.
Masoumeh Ezati
Amir Hashemi
Larisa Chmelíková, Ph.D.
Minoo Partovi Nasr
Ing. Inna Zumberg

Syllabus

1. Cell engineering - cultivation, observation
2. TEER measurement of cultivated cells
3. Observation techniques, fluroescence microscopy
4. Cells segmentation (image analysis)
5. Cells migrations, data processing
6. Signals processing MEA+AFM
7. Fusion 360 basics
8. 3D printing in cardiology, segmentation (Slicer)
9. Biomaterials preparation
10. 3D bioprinting: practical task (Inkredible)
11. Virtual Reality for Fusion models
12. Excursion  

eLearning

eLearning: currently opened course