Course detail

Concepts of Biofotonics

FSI-9VKBAcad. year: 2023/2024

The course provides an overview of modern techniques using electromagnetic radiation suitable for studying the structure, function and dynamics of biological macro and micro objects and of methods applicable to the detection, diagnosis and treatment of diseases in a non-invasive way.

Language of instruction


Number of ECTS credits


Mode of study

Not applicable.

Entry knowledge

Knowledge of geometric, wave and physical optics.

Rules for evaluation and completion of the course

Doctoral exam in the presented subject extent examining in particular the understanding of the physical nature of biophotonics methods.
Attendance at the lecture is optional.


The aim is to understand the principles of application of photonics in specific areas of biology and medicine.
Knowledge of physical and optical principles of biophotonics, which gives a deeper basis for its practical use.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

P. N. Prasad: “Introduction to Biophotonics”, John Wiley & Sons, Inc., 2003. (EN)
J. B. Pawley: “Handbook of Biological Confocal Microscopy”, 2nd Edition, Plenum Press, 1995. (EN)
Krafft, C: Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs. J Biophotonics 9 (2016) 1362-1375. (EN)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme D-FIN-K Doctoral, 1. year of study, winter semester, recommended
  • Programme D-FIN-P Doctoral, 1. year of study, winter semester, recommended

Type of course unit



20 hours, optionally

Teacher / Lecturer


1. Recapitulation of electromagnetic radiation theory.
2. Sources of radiation, lasers.
3. Interaction of radiation with matter, effect of radiation on tissues.
4. Basic methods of optical microscopy.
5. Confocal microscopy, OCT / OCM, holographic microscopy.
6. Fluorescence microscopy, Raman microscopy.
7. Flow cytometry.
8. Optical manipulation with microobjects, application of lasers in medicine.
9. Photodynamic therapy, optical biosensors.
10. Application of micro- and nano-opto-technologies in medicine (retinal replacements).