Course detail

Experimental Biophotonics

FSI-TEB-AAcad. year: 2023/2024

Basics of cell biology in vitro: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopical observations. Protocols of in vitro investigations of cell behaviour. Interpretation and evaluation of results of microscopical observations of cell behaviour. The role of light microscopy techniques for study of live cells in vitro: principles of light microscopy, fluorescence and confocal microscopy, quantitative phase imaging in holographic microscopy, image processing and analysis.

Demonstrations and practical sections in the laboratory.

Language of instruction

English

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

doc. Ing. Daniel Zicha, CSc.

Department

Institute of Physical Engineering (ÚFI)

Entry knowledge

Knowledge of elements of geometric optics.

Rules for evaluation and completion of the course

Student assesment will take into consideration his/her work at laboratory and wiil depend on the result of final discussion on specified themes. Notes and literature is permitted.
Presence in all teaching units is required because of their mutual links. In the case of student absention, tacher will specify alternative requirements.

Aims

Objective of the theme is the introduction into the basics and prospective of live cell biology in vitro as observed and studied with techniques of light microscopy.
Acquaintance with the basics of live cell biology in vitro; preparation and cultivation of cell for microscopy observation; application of light microscopies to study cell living in vitro; elements of interpretation of results of biological experiments from physical and biological points of view.
Understanding problems and gaining insight into current trends of biological microscopy.
Acquiring of widened ability to interdisciplinary professional activity.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

A. R. Hibbs: Confocal Microscopy for Biologists. Springer, 2004.
B. Alberts et al.: Molecular Biology of the Cell, 5th Ed. Garland Science, 2007.
D. Bray: Cell Movements: From Molecules to Motility. Garland Science 2000.
D. E. Chandler, R. W. Robertson: Bioimaging. Jones & Bartlett, Boston 2009.

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme N-FIN-P Master's 1 year of study, winter semester, elective
  • Programme N-PMO-P Master's 1 year of study, winter semester, elective

Type of course unit

 

Lecture

13 hod., optionally

Teacher / Lecturer

doc. Ing. Daniel Zicha, CSc.

Syllabus

Structure and functions of the cell
Cells isolation into in vitro systems
Principles of light microscopy
Mastering the specialty of preparation of live cells for microscopical observations
Protocols of in vitro investigations of cell behaviour.
Fluorescence and confocal microscopy
Quantitative phase contrast in holographic microscopy
Image processing and analysis
Analysis of results of biological experiments

Laboratory exercise

13 hod., compulsory

Teacher / Lecturer

doc. Ing. Daniel Zicha, CSc.

Syllabus

Lecture themes are developed by laboratory demonstrations and laboratory work of students.