Course detail

Technology in Cellular Engineering

FEKT-MPA-TBIAcad. year: 2022/2023

Not applicable.

Language of instruction

English

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

Ing. Vratislav Čmiel, Ph.D.

Department

Department of Biomedical Engineering (UBMI)

Offered to foreign students

The home faculty only

Learning outcomes of the course unit

Not applicable.

Prerequisites

Knowledge of biology, mathematics and physics accordant with the subjects of previous bachelor’s study is expected.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Not applicable.

Course curriculum

1. Cultivation and preparation of cells and tissue, automation of processes in the cell laboratory.
2. Technology for cell counting and sorting, automated laboratory analyzers for tissue engineering.
3. Mechanical properties and methods of their measurement. Holographic microscopy and its applications.
4. Microscopic techniques, light field observation, contrast techniques, fluorescence microscopy.
5. Special microscopic imaging techniques in cell engineering. Recording techniques for microscopy.
6. Electron microscopy in cell engineering, correlation microscopy, preparation of biological samples for electron microscopy.
7. Methods for measuring electrical activity of excitable tissue using microelectrode arrays and microelectrode systems.
8. Methods and technologies for cell migration analysis.
9. Nanotechnology in regenerative and cancer medicine.
10. 3D bioprinting and its application in regenerative medicine.
11. Technologies and methods used in optogenetics.
12. Microfluidic systems and their use in cell engineering.

Work placements

Not applicable.

Aims

Not applicable.

Specification of controlled education, way of implementation and compensation for absences

Not applicable.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Alberts B., Johnson A., Walter P.: Molecular Biology of the Cell. (CS)
José Luis R. Arrondo, Alicia Alonso: Advanced Techniques in Biophysics. (CS)
Bengt Nölting: Methods in Modern Biophysics (CS)
Ankur Singh, Akhilesh K. Gaharwar: Microscale Technologies for Cell Engineering (CS)