English

5

Not applicable.

Students will understand the biosensors: their fundamentals, designs, types and most recent impactful applications.

The basic knowledge of biochemistry and electrochemistry are required.

Not applicable.

Lectures and practical course.

Requirements for the successful completion of the subject are set by an updated regulation of the guarantor.
Lectures: oral exam
Practical course: protocols and test

Lectures:
1. Introduction and history of biosensors, classification, applications. Characteristics of biosensors.
2. Introduction to electronics.
3. Electrochemical biosensors - introduction, types of electrodes, two- and multi-electrode circuits. Ampereometric measurement of oxygen, hydrogen peroxide and NADH, oxidase and dehydrogenase based biosensors.
4. Mediators of electron transfer. Potentiometric biosensors, ISFET, LAPS, voltammetric techniques.
5. Conductometric biosensors and impedance measurement.
6. Calorimetric biosensors and piezoelectric biosensors.
7. Optical biosensors - direct and indirect measurement principles; luminescence, optical fibers, SPR technology.
8. Enzyme biosensors, whole cell biosensors.
9. Affinity biosensors - immunosensors and nucleic acid biosensors.
10. Methods of biomolecule immobilization - physical and chemical immobilization.
11. Nanoparticles and nanostructured surfaces in biosensors.
12. Production and miniaturization of sensors. Screen printing, lithography, biochips.
13. Fundamentals of microfluidics, lab-on-chip, commercial biosensors.

Practical course:
1. Impedance measurement of bioaffinity interaction
2. Conductivity and pH measurement
3. Measurement of oxygen
4. Electrochemical measurement of heavy metal in solutions
5. Electrochemical measurement of glucose and ascorbic acid using carbon electrode
6. Amperometric enzymatic biosensor for dection of glucose
7. Credit test

Not applicable.

The objective of course is to provide an overview of biosensors including the physico-chemical theory, construction and application in clinical analysis, environment, food and safety areas.
Students will understand the basics of biorecognition events (bioaffinity, biocatalytic) and get knowledge in biosensor technologies (lithography, miniaturization, microfluidics), immobilization procedures and application of nanomaterials (nanoparticles, nanowires) in biosensing.

Specification of the course and its implementation is specified by guarantor’s regulation updated for every academic year.

Not applicable.

Not applicable.

Biosensors and Biodetection: Methods and Protocols, ISBN 978-1-4939-6911-1 (EN)
Jeong-Yeol Yoon: Introduction to Biosensors: From Electric Circuits to Immunosensors (EN)

Not applicable.

26 hours, compulsory