Course detail

Electronics and Biosensors

FEKT-MPA-BSNAcad. year: 2022/2023

The concept of the course is focused on the fundamental aspects of biosensors and electronic biochips. Students will gain an overview of biosensors, especially of electrochemical, optical, piezoelectric and calorimetric type. They will get knowlegde about biocatalytic (enzymes) and bioaffinity (antigen-antibody, DNA or biomimetic) biosensors and their application in medicine, environment and food area. A part of the course will be devoted to the sensor technology (lithography, miniaturization, microfluidics) and nanomaterial (nanoparticles, nanowires) application in biosensing.

Language of instruction


Number of ECTS credits


Mode of study

Not applicable.

Learning outcomes of the course unit

Students will understand the biosensors: their fundamentals, designs, types and most recent impactful applications.


The basic knowledge of biochemistry and electrochemistry are required.


Not applicable.

Planned learning activities and teaching methods

Lectures and practical course.

Assesment methods and criteria linked to learning outcomes

Requirements for the successful completion of the subject are set by an updated regulation of the guarantor.
Lectures: oral exam
Practical course: protocols and test

Course curriculum

1. Introduction and history of biosensors, classification, applications. Characteristics of biosensors.
2. Introduction to electronics.
3. Electrochemical biosensors - introduction, types of electrodes, two- and multi-electrode circuits. Ampereometric measurement of oxygen, hydrogen peroxide and NADH, oxidase and dehydrogenase based biosensors.
4. Mediators of electron transfer. Potentiometric biosensors, ISFET, LAPS, voltammetric techniques.
5. Conductometric biosensors and impedance measurement.
6. Calorimetric biosensors and piezoelectric biosensors.
7. Optical biosensors - direct and indirect measurement principles; luminescence, optical fibers, SPR technology.
8. Enzyme biosensors, whole cell biosensors.
9. Affinity biosensors - immunosensors and nucleic acid biosensors.
10. Methods of biomolecule immobilization - physical and chemical immobilization.
11. Nanoparticles and nanostructured surfaces in biosensors.
12. Production and miniaturization of sensors. Screen printing, lithography, biochips.
13. Fundamentals of microfluidics, lab-on-chip, commercial biosensors.

Practical course:
1. Impedance measurement of bioaffinity interaction
2. Conductivity and pH measurement
3. Measurement of oxygen
4. Electrochemical measurement of heavy metal in solutions
5. Electrochemical measurement of glucose and ascorbic acid using carbon electrode
6. Amperometric enzymatic biosensor for dection of glucose
7. Credit test

Work placements

Not applicable.


The objective of course is to provide an overview of biosensors including the physico-chemical theory, construction and application in clinical analysis, environment, food and safety areas.
Students will understand the basics of biorecognition events (bioaffinity, biocatalytic) and get knowledge in biosensor technologies (lithography, miniaturization, microfluidics), immobilization procedures and application of nanomaterials (nanoparticles, nanowires) in biosensing.

Specification of controlled education, way of implementation and compensation for absences

Specification of the course and its implementation is specified by guarantor’s regulation updated for every academic year.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Biosensors and Biodetection: Methods and Protocols, ISBN 978-1-4939-6911-1 (EN)
Jeong-Yeol Yoon: Introduction to Biosensors: From Electric Circuits to Immunosensors (EN)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme MPA-MEL Master's, any year of study, winter semester, elective
  • Programme MPAD-MEL Master's, any year of study, winter semester, elective

Type of course unit



26 hours, optionally

Teacher / Lecturer

Laboratory exercise

26 hours, compulsory

Teacher / Lecturer