The content of the course is to acquaint students with the principles and reasons for testing of chemicals, chemical products and wastes with regard to their potential ecotoxicity and to familiarize with the practical utility of the obtained results in the assessment of the effects of tested matrices on ecosystems. Structure of content is following. Ecological properties of chemicals, chemical products and waste. Basic principles and reasons for testing of chemicals and wastes. Legislation in the field ecotoxicological testing of chemicals and waste, sludge and sediments. Importance and the main principles of aquatic and terrestrial ecotoxicology, Bioassays - definition, importance, principles, classification. Preparation of chemicals for ecotoxicological testing; testing scheme, the concentration range, limit, preliminary and final tests. Preparation of waste water leachates for the purposes of assessing hazardous properties ecotoxicity H-14 (initial tests, confirmatory tests, preliminary tests, basic tests). Testing and subsequent evaluation of the results of tests conducted on organisms of aquatic ecosystems (Daphnia magna, Thamnocephalus platyurus, Lemna minor, Vibrio fischeri, Desmodesmus subspicatus). Testing and evaluation of test results on organisms of terrestrial ecosystems (Eisenia fetida, Sinapis alba, Lactuca sativa).