Course detail

Chromatography

FCH-MC_CHRAcad. year: 2011/2012

History of chromatography. Basic physical-chemical principles of chromatography. Liquid chromatography - individual techniques based on different separation mechanisms, instrumentation, applications, current trends. Gas chromatography - individual techniques based on different separation mechanisms, instrumentation, applications, current trends. Interpretation of chromatographic data, qualitative and quantitative analysis.

Language of instruction

Czech

Number of ECTS credits

3

Mode of study

Not applicable.

Guarantor

prof. Ing. Josef Čáslavský, CSc.

Department

Institute of Chemistry and Technology of Environmental Protection (ÚCHTOŽP)

Learning outcomes of the course unit

Graduate obtains knowledge about the theory of chromatographic separation techniques, about the instrumentation of liquid and gas chromatography, about their application in analytical praxis. He/She will get ability to select the most suitable chromatographic technique for the solution of real analytical tass and to critically evaluate obtained data.

Prerequisites

Thorough knowledge of physical chemistry, good knowledge of common, inorganic, organic and analytical chemistry.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

Oral examination covering the range of discussed topics

Course curriculum

1. Introduction, history of chromatography, classification of chromatographic techniques
2. Basic theory of chromatography – thermodynamical and kinetic aspects, effectivity of chromatographic separation
3. Planar chromatography
4. Column chromatography – basic configuration and theory
5. High performance liquid chromatography – separation principles, instrumentation, columns and rules of their selection, normal and reversed-phase systems, isocratic and gradient elution, ion chromatography, ion-pair chromatography, hydrophobic interaction liquid chromatography, size exclusion chromatography, bioaffinity chromatography, current trends – UPLC, UHPLC
6. Gas chromatography – separation principles, instrumentation, capillary and packed columns, rules for their selection, classification of stationary phases in gas chromatography, derivatization in gas chromatography
7. Tandem techniques in chromatography – systems LC-LC, LC-GC, GC-GC, GCxGC
8. Qualitative aspects of chromatographic data – correlation structure-retention, retention indices in gas chromatography
9. Quantitation in chromatography

Work placements

Not applicable.

Aims

Thorough familiarization of students with chromatographic separation techniques and with application of chromatography including interpretation of chromatographic data

Specification of controlled education, way of implementation and compensation for absences

Not applicable.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Churáček J. a kol.: Analytická separace látek. STNL Praha, 1990 (CS)
Churáček J. a kol.: Nové trendy v teorii a instrumentaci vybraných analytických metod. SNTL Praha, 1993. (CS)
V. R. Meyer: Practical High-Performance. Liquid Chromatography, 2004 (EN)

Recommended reading

Not applicable.

Classification of course in study plans

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

prof. Ing. Josef Čáslavský, CSc.