Publication result detail

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

ČMIEL, V.; SKOPALÍK, J.; BAIAZITOVA, L.; PROVAZNÍK, I.

Original Title

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

English Title

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

The main aim of the study was to use the spectral excitation-emission 2D scanning followed by 2D spectral analysis in order to obtain an appropriate representation of the properties of the used dye in the original image of a stained cell. We used Di-4-ANNEPS voltage sensitive dye (Invitrogen) binding to an adult rat cardiomyocyte membrane to determine the stability of the dye and to characterize spatial differences of its spectral properties.

English abstract

The main aim of the study was to use the spectral excitation-emission 2D scanning followed by 2D spectral analysis in order to obtain an appropriate representation of the properties of the used dye in the original image of a stained cell. We used Di-4-ANNEPS voltage sensitive dye (Invitrogen) binding to an adult rat cardiomyocyte membrane to determine the stability of the dye and to characterize spatial differences of its spectral properties.

Keywords

confocal microscopy; fluorescence; spectral scan; Stokes shift

Key words in English

confocal microscopy; fluorescence; spectral scan; Stokes shift

Authors

ČMIEL, V.; SKOPALÍK, J.; BAIAZITOVA, L.; PROVAZNÍK, I.

RIV year

2020

Released

05.08.2019

Publisher

Cambridge University Press

Location

New York

ISBN

1435-8115

Periodical

MICROSCOPY AND MICROANALYSIS

Volume

25

Number

Suppl 2

State

United States of America

Pages from

1264

Pages to

1265

Pages count

2

URL

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/9BF44630933F77D35CE7544BF48EA85D/S1431927619007050a.pdf/method_for_analysis_of_excitationemission_spectral_scans_to_characterize_fluorescent_probes_in_confocal_microscopy.pdf

BibTex

@article{BUT157958,
  author="Vratislav {Čmiel} and Josef {Skopalík} and Larisa {Chmelíková} and Valentýna {Provazník}",
  title="Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy",
  journal="MICROSCOPY AND MICROANALYSIS",
  year="2019",
  volume="25",
  number="Suppl 2",
  pages="1264--1265",
  doi="10.1017/S1431927619007050",
  issn="1431-9276",
  url="https://www.cambridge.org/core/services/aop-cambridge-core/content/view/9BF44630933F77D35CE7544BF48EA85D/S1431927619007050a.pdf/method_for_analysis_of_excitationemission_spectral_scans_to_characterize_fluorescent_probes_in_confocal_microscopy.pdf"
}