Detail publikačního výsledku

All-in-one detector of circulating mRNA based on a smartphone

ČMIEL, V.; GUMULEC, J.; SVOBODA, O.; RAUDENSKÁ, M.; HUDCOVÁ, K.; SEKORA, J.; BALOGH, J.; MASAŘÍK, M.; PROVAZNÍK, I.

Original Title

All-in-one detector of circulating mRNA based on a smartphone

English Title

All-in-one detector of circulating mRNA based on a smartphone

Type

Paper in proceedings (conference paper)

Original Abstract

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

English abstract

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Keywords

mRNA, smartphone, PCR, cancer, metallothionein

Key words in English

mRNA, smartphone, PCR, cancer, metallothionein

Authors

ČMIEL, V.; GUMULEC, J.; SVOBODA, O.; RAUDENSKÁ, M.; HUDCOVÁ, K.; SEKORA, J.; BALOGH, J.; MASAŘÍK, M.; PROVAZNÍK, I.

RIV year

2017

Released

04.03.2016

Publisher

SPIE

Location

San Francisco

ISBN

9781628419498

Book

Proceedings of SPIE

Pages from

1

Pages to

6

Pages count

6

URL

http://dx.doi.org/10.1117/12.2213253

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@inproceedings{BUT122889,
  author="Vratislav {Čmiel} and Jaromír {Gumulec} and Ondřej {Svoboda} and Martina {Raudenská} and Kristýna {Hudcová} and Jiří {Sekora} and Jaroslav {Balogh} and Michal {Masařík} and Valentýna {Provazník}",
  title="All-in-one detector of circulating mRNA based on a smartphone",
  booktitle="Proceedings of SPIE",
  year="2016",
  number="9715",
  pages="1--6",
  publisher="SPIE",
  address="San Francisco",
  doi="10.1117/12.2213253",
  isbn="9781628419498",
  url="http://dx.doi.org/10.1117/12.2213253"
}