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ŠPANOVÁ, A., DRÁB, V., TURKOVÁ, K., ŠPANO, M., BURDYCHOVÁ, R., ŠEDO, O., ŠRŮTKOVÁ, D., RADA, V., RITTICH, B.
Original Title
Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry.
Type
journal article in Web of Science
Language
English
Original Abstract
Phenotypic and genotypic methods and MALDI-TOF mass spectrometry were applied for the species identification of 29 out of 30 strains of the genus Lactobacillus isolated from faecal samples of 30 breastfed infants. All strains were tested for growth in milk and/or reconstituted skimmed milk supplemented with 0.5 % yeast extract, production of lactic acid, bile salts and low pH tolerance, and for production of β-glucuronidase. PCR screenings of tyrosine-decarboxylase and histidine-decarboxylase genes were also carried out. Survival of Lactobacillus cells at low pH and their bile tolerance were strain-specific. Finally, seven strains of probiotic species were selected for possible use in the dairy industry and for further tests concerning their probiotic characteristics.
Keywords
Lactobacillus; identification; MALDI-TOF MS; in vitro tests; probiotic characteristiky
Authors
RIV year
2015
Released
31. 7. 2015
Publisher
Springer
Location
Spolková republika Německo
ISBN
1438-2377
Periodical
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
Year of study
241
Number
6
State
Federal Republic of Germany
Pages from
861
Pages to
869
Pages count
9
URL
hptt://springer.com
BibTex
@article{BUT115365, author="Bohuslav {Rittich} and Alena {Španová} and Kristýna {Turková} and Miroslav {Špano}", title="Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry.", journal="EUROPEAN FOOD RESEARCH AND TECHNOLOGY", year="2015", volume="241", number="6", pages="861--869", doi="10.1007/s00217-015-2511-1", issn="1438-2377", url="hptt://springer.com" }