Publication detail

Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry.

ŠPANOVÁ, A., DRÁB, V., TURKOVÁ, K., ŠPANO, M., BURDYCHOVÁ, R., ŠEDO, O., ŠRŮTKOVÁ, D., RADA, V., RITTICH, B.

Original Title

Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry.

Type

journal article in Web of Science

Language

English

Original Abstract

Phenotypic and genotypic methods and MALDI-TOF mass spectrometry were applied for the species identification of 29 out of 30 strains of the genus Lactobacillus isolated from faecal samples of 30 breastfed infants. All strains were tested for growth in milk and/or reconstituted skimmed milk supplemented with 0.5 % yeast extract, production of lactic acid, bile salts and low pH tolerance, and for production of β-glucuronidase. PCR screenings of tyrosine-decarboxylase and histidine-decarboxylase genes were also carried out. Survival of Lactobacillus cells at low pH and their bile tolerance were strain-specific. Finally, seven strains of probiotic species were selected for possible use in the dairy industry and for further tests concerning their probiotic characteristics.

Keywords

Lactobacillus; identification; MALDI-TOF MS; in vitro tests; probiotic characteristiky

Authors

ŠPANOVÁ, A., DRÁB, V., TURKOVÁ, K., ŠPANO, M., BURDYCHOVÁ, R., ŠEDO, O., ŠRŮTKOVÁ, D., RADA, V., RITTICH, B.

RIV year

2015

Released

31. 7. 2015

Publisher

Springer

Location

Spolková republika Německo

ISBN

1438-2377

Periodical

EUROPEAN FOOD RESEARCH AND TECHNOLOGY

Year of study

241

Number

6

State

Federal Republic of Germany

Pages from

861

Pages to

869

Pages count

9

URL

hptt://springer.com

BibTex

@article{BUT115365,
  author="Bohuslav {Rittich} and Alena {Španová} and Kristýna {Turková} and Miroslav {Špano}",
  title="Selection of potential probiotic Lactobacillus strains of human origin
for use in dairy industry.",
  journal="EUROPEAN FOOD RESEARCH AND TECHNOLOGY",
  year="2015",
  volume="241",
  number="6",
  pages="861--869",
  doi="10.1007/s00217-015-2511-1",
  issn="1438-2377",
  url="hptt://springer.com"
}