Publication result detail

DNA based identification of microorganisms in a probiotic products

ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.

Original Title

DNA based identification of microorganisms in a probiotic products

English Title

DNA based identification of microorganisms in a probiotic products

Type

Abstract

Original Abstract

Brewers yeasts (Saccharomyces cerevisiae) are a secondary product from production of beer. They are used as a nutritional supplement for long time, too. Brewers yeasts can be supplemented with probiotic bacteria and roughage and with vitamins, minerals and enzymes, too. Brewers yeast tablets with content of genus Lactobacillus and Biffidobacterium were used for analysis. Two types of samples were before expiration, and other were after expiration (1 and 8 years). DNA was isolated from crude cell lysates of brewers yeast tablets using magnetics P(HEMA-co-GMA) microspheres covered by carboxyl groups. Non-diluted DNA was used for analysis of Lactobacillus and Biffidobacterium. The conventional polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) were used for amplification of DNAs.

English abstract

Brewers yeasts (Saccharomyces cerevisiae) are a secondary product from production of beer. They are used as a nutritional supplement for long time, too. Brewers yeasts can be supplemented with probiotic bacteria and roughage and with vitamins, minerals and enzymes, too. Brewers yeast tablets with content of genus Lactobacillus and Biffidobacterium were used for analysis. Two types of samples were before expiration, and other were after expiration (1 and 8 years). DNA was isolated from crude cell lysates of brewers yeast tablets using magnetics P(HEMA-co-GMA) microspheres covered by carboxyl groups. Non-diluted DNA was used for analysis of Lactobacillus and Biffidobacterium. The conventional polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) were used for amplification of DNAs.

Keywords

probiotic products, polymerase chain reaction, real time polymerase chain reaction, DNA isolation

Key words in English

probiotic products, polymerase chain reaction, real time polymerase chain reaction, DNA isolation

Authors

ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.

RIV year

2014

Released

28.05.2013

Publisher

Institute of Immunology and Experimental Therapy Polish academy of Science

Location

Kudowa Zdrój, Polsko

ISBN

978-83-928488-3-7

Book

Microbiology and Immunology of Mucosa Probiotics Conference 2013

Edition

1

Pages from

41

Pages to

41

Pages count

1

BibTex

@misc{BUT99950,
  author="Martina {Čakajdová} and Štěpánka {Trachtová} and Alena {Španová} and Daniel {Horák} and Bohuslav {Rittich}",
  title="DNA based identification of microorganisms in a probiotic products",
  booktitle="Microbiology and Immunology of Mucosa Probiotics Conference 2013",
  year="2013",
  series="1",
  edition="1",
  pages="41--41",
  publisher="Institute of Immunology and Experimental Therapy Polish academy of Science",
  address="Kudowa Zdrój, Polsko",
  isbn="978-83-928488-3-7",
  note="Abstract"
}