Detail publikačního výsledku

Electrochemical fabrication of optical biosensor for DNA detection

VALKOVIČOVÁ, J.; HRDÝ, R.; DRBOHLAVOVÁ, J.; HUBÁLEK, J.

Original Title

Electrochemical fabrication of optical biosensor for DNA detection

English Title

Electrochemical fabrication of optical biosensor for DNA detection

Type

Paper in proceedings (conference paper)

Original Abstract

The work is focused on quantum dots (QDs) array fabrication from non-toxic titanium dioxide using template based non-lithographic method, their luminescence properties study and finally their usage for DNA optical detection in situ. The paper describes TiO2 QDs surface modification with gold using suitable template technique and subsequent functionalization of gold modified QDs surface via avidin-biotin molecules and further with single stranded DNA (ssDNA) or oligonucleotides. This functinalization step provides a suitable base for subsequent hybridization reaction with detected nucleic acid. The system of biosensor (TiO2 QDs modified with gold and biomolecules) is designed for rapid optical diagnosis of viral diseases.

English abstract

The work is focused on quantum dots (QDs) array fabrication from non-toxic titanium dioxide using template based non-lithographic method, their luminescence properties study and finally their usage for DNA optical detection in situ. The paper describes TiO2 QDs surface modification with gold using suitable template technique and subsequent functionalization of gold modified QDs surface via avidin-biotin molecules and further with single stranded DNA (ssDNA) or oligonucleotides. This functinalization step provides a suitable base for subsequent hybridization reaction with detected nucleic acid. The system of biosensor (TiO2 QDs modified with gold and biomolecules) is designed for rapid optical diagnosis of viral diseases.

Keywords

quantum dots, quenching, BSA

Key words in English

quantum dots, quenching, BSA

Authors

VALKOVIČOVÁ, J.; HRDÝ, R.; DRBOHLAVOVÁ, J.; HUBÁLEK, J.

RIV year

2013

Released

30.05.2012

Publisher

Mendelova univerzita v Brně

Location

Brno

ISBN

978-80-7375-618-5

Book

XII. Pracovní setkání fyzikálních chemiků a elektrochemiků

Pages from

314

Pages to

316

Pages count

2

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@inproceedings{BUT93844,
  author="Jiřina {Valkovičová} and Radim {Hrdý} and Jana {Drbohlavová} and Jaromír {Hubálek}",
  title="Electrochemical fabrication of optical biosensor for DNA detection",
  booktitle="XII. Pracovní setkání fyzikálních chemiků a elektrochemiků",
  year="2012",
  number="1.",
  pages="314--316",
  publisher="Mendelova univerzita v Brně",
  address="Brno",
  isbn="978-80-7375-618-5"
}