Square wave voltammetry as a tool for study of ellipticine-DNA interaction

Square wave voltammetry as a tool for study of ellipticine-DNA interaction

Abstract

Ellipticine and its derivates belong to prototype compounds acting predominantly by DNA-damaging mechanisms and serving as cytostaic agents. The aim of this work was to study the intercalation of the ellipticine into DNA. For this purpose we utilized adsorptive transfer stripping technique (AdTS) coupled with square wave voltammetry (SWV). Primarily, we investigated the influence of ellipticine on genomic DNA electrochemical signals. Decrease in redox signal of cytosine/adenine (at -1.4 V) was observed as a result of ellipticine intercalation into DNA. The decrease was proportional to increasing concentration of ellipticine.

Ellipticine and its derivates belong to prototype compounds acting predominantly by DNA-damaging mechanisms and serving as cytostaic agents. The aim of this work was to study the intercalation of the ellipticine into DNA. For this purpose we utilized adsorptive transfer stripping technique (AdTS) coupled with square wave voltammetry (SWV). Primarily, we investigated the influence of ellipticine on genomic DNA electrochemical signals. Decrease in redox signal of cytosine/adenine (at -1.4 V) was observed as a result of ellipticine intercalation into DNA. The decrease was proportional to increasing concentration of ellipticine.

Ellipticine, DNA

Ellipticine, DNA

MASAŘÍK,M.; HÚSKA,D.; ADAM,V.; HUBALEK,J.; PROVAZNÍK, I.; TRNKOVÁ,L.; ECKSCHLAGER,T.; STIBOROVÁ,M.; KIZEK,R.

2013

01.01.2010

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

1107-3756

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

26

1

United States of America

S46

S46

1