Publication result detail

. Optimization of lipase production by Bacillus Optimization of lipase production by Bacillus subtilis

VOBĚRKOVÁ, S.; HERMANOVÁ, S.; OMELKOVÁ, J.; SLAVÍČKOVÁ, R.; RICHTERA, L.

Original Title

. Optimization of lipase production by Bacillus Optimization of lipase production by Bacillus subtilis

English Title

. Optimization of lipase production by Bacillus Optimization of lipase production by Bacillus subtilis

Type

Abstract

Original Abstract

The aim of this work was to study the effect of pH and temperature on the activity of lipase produced by the submerged culture of Bacillus subtilis CCM 1999. A series of cultivation media differing in the carbon sources and the nutrient content; brain heart infusion broth, nutrient broth and nutrient broth supplemented with 2 % glucose was used. The activity of lipase was measured spectrophotometrically using p-nitrophenyl laurate in ethanol as the substrate.

English abstract

The aim of this work was to study the effect of pH and temperature on the activity of lipase produced by the submerged culture of Bacillus subtilis CCM 1999. A series of cultivation media differing in the carbon sources and the nutrient content; brain heart infusion broth, nutrient broth and nutrient broth supplemented with 2 % glucose was used. The activity of lipase was measured spectrophotometrically using p-nitrophenyl laurate in ethanol as the substrate.

Keywords

Bacillus subtilis

Key words in English

Bacillus subtilis

Authors

VOBĚRKOVÁ, S.; HERMANOVÁ, S.; OMELKOVÁ, J.; SLAVÍČKOVÁ, R.; RICHTERA, L.

RIV year

2012

Released

17.08.2011

Publisher

Česká společnost chemická

Location

Praha

ISBN

0009-2770

Periodical

CHEMICKE LISTY

Volume

105 (S)

Number

Symposia

State

Czech Republic

Pages from

s1003

Pages to

s1004

Pages count

1