Publication result detail

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

HÚSKA, D.; ADAM, V.; BABULA, P.; TRNKOVÁ, L.; HUBÁLEK, J.; ZEHNÁLEK, J.; HAVEL, L.; KIZEK, R.

Original Title

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

English Title

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

English abstract

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

Keywords

Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles

Key words in English

Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles

Authors

HÚSKA, D.; ADAM, V.; BABULA, P.; TRNKOVÁ, L.; HUBÁLEK, J.; ZEHNÁLEK, J.; HAVEL, L.; KIZEK, R.

RIV year

2013

Released

01.04.2011

Publisher

SPRINGER WIEN,

ISBN

0026-3672

Periodical

MICROCHIMICA ACTA

Volume

2012 (173)

Number

1-2

State

Republic of Austria

Pages from

189

Pages to

197

Pages count

9

BibTex

@article{BUT75745,
  author="HÚSKA, D. and ADAM, V. and BABULA, P. and TRNKOVÁ, L. and HUBÁLEK, J. and ZEHNÁLEK, J. and HAVEL, L. and KIZEK, R.",
  title="Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA",
  journal="MICROCHIMICA ACTA",
  year="2011",
  volume="2012 (173)",
  number="1-2",
  pages="189--197",
  issn="0026-3672"
}