Publication result detail

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients

ZÍTKA, O.; KŘÍŽKOVÁ, S.; KREJČOVÁ, L.; HYNEK, D.; GUMULEC, J.; MASAŘÍK, M.; SOCHOR, J.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KIZEK, R.

Original Title

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients

English Title

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients

Type

WoS Article

Original Abstract

A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as lab on valve. The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.

English abstract

A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as lab on valve. The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.

Keywords

Electrochemistry, ELISA, Magnetic particles, Nanoseparation

Key words in English

Electrochemistry, ELISA, Magnetic particles, Nanoseparation

Authors

ZÍTKA, O.; KŘÍŽKOVÁ, S.; KREJČOVÁ, L.; HYNEK, D.; GUMULEC, J.; MASAŘÍK, M.; SOCHOR, J.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KIZEK, R.

RIV year

2012

Released

01.09.2011

Publisher

Wiley

ISBN

0173-0835

Periodical

ELECTROPHORESIS

Volume

2011 (32)

Number

1

State

Federal Republic of Germany

Pages from

3207

Pages to

3220

Pages count

14

BibTex

@article{BUT75097,
  author="Ondřej {Zítka} and Soňa {Křížková} and Ludmila {Krejčová} and David {Hynek} and Jaromír {Gumulec} and Michal {Masařík} and Jiří {Sochor} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and René {Kizek}",
  title="Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients",
  journal="ELECTROPHORESIS",
  year="2011",
  volume="2011 (32)",
  number="1",
  pages="3207--3220",
  doi="10.1002/elps.201100430",
  issn="0173-0835"
}