Publication result detail

Phytochelatin synthase activity as a marker of metal pollution

ZÍTKA, O.; KRYŠTOFOVÁ, O.; ŠOBROVÁ, P.; ADAM, V.; ZEHNÁLEK, J.; BEKLOVÁ, M.; KIZEK, R.

Original Title

Phytochelatin synthase activity as a marker of metal pollution

English Title

Phytochelatin synthase activity as a marker of metal pollution

Type

WoS Article

Original Abstract

The synthesis of phytochelatins is catalyzed by gamma-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO3)2 for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35 C for 30 min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of PCS (270 fkat) in treated cells was more than seven times higher in comparison to control ones. Km for PCS was estimated as 2.3 mM.

English abstract

The synthesis of phytochelatins is catalyzed by gamma-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO3)2 for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35 C for 30 min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of PCS (270 fkat) in treated cells was more than seven times higher in comparison to control ones. Km for PCS was estimated as 2.3 mM.

Keywords

Phytochelatin synthase activity, Cadmium, Glutathione, High performance liquid chromatography with electrochemical detection, Tobacco BY-2 cell suspension

Key words in English

Phytochelatin synthase activity, Cadmium, Glutathione, High performance liquid chromatography with electrochemical detection, Tobacco BY-2 cell suspension

Authors

ZÍTKA, O.; KRYŠTOFOVÁ, O.; ŠOBROVÁ, P.; ADAM, V.; ZEHNÁLEK, J.; BEKLOVÁ, M.; KIZEK, R.

RIV year

2012

Released

02.06.2011

Publisher

Elsevier

ISBN

0304-3894

Periodical

JOURNAL OF HAZARDOUS MATERIALS

Volume

2011 (192)

Number

1

State

Kingdom of the Netherlands

Pages from

794

Pages to

800

Pages count

7

BibTex

@article{BUT75096,
  author="Ondřej {Zítka} and Olga {Kryštofová} and Pavlína {Adam} and Vojtěch {Adam} and Josef {Zehnálek} and Miroslava {Beklová} and René {Kizek}",
  title="Phytochelatin synthase activity as a marker of metal pollution",
  journal="JOURNAL OF HAZARDOUS MATERIALS",
  year="2011",
  volume="2011 (192)",
  number="1",
  pages="794--800",
  doi="10.1016/j.jhazmat.2011.05.088",
  issn="0304-3894"
}