Publication result detail

Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein

ZÍTKA, O.; HUBÁLEK, J.; KŘÍŽKOVÁ, S.; HÚSKA, D.; ADAM, V.; ECKSCHLAGER, T.; KIZEK, R.

Original Title

Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein

English Title

Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

Matrix metalloproteinases (MMPs) play numerous roles in physiological and pathological processes including cancer. Interactions of MMPs with other zinc-binding proteins are of great interest mainly from the point of view of the participation of these proteins in carcinogenesis. The aim of this study was to utilize chip gel electrophoresis for investigation of matrix metalloproteinase 9 (gelatinase B, MMP-9) interactions with collagen. The interaction was observed and the effect of temperature was tested. We further focused on the study of activation of MMP-9 by the presence of zinc-binding proteins called metallothioneins (MT). We confirmed the basic presumption of the activation of MMP-9 by the presence of MT similar to the temperature effect. Moreover, we chose the method of differential pulse voltammetry Brdicka reaction to verify our results. It can be concluded that the activity of MMP-9 was higher in the presence of MT in comparison with the experimental collagen(type I)-MMP-9 mixture in vitro.

English abstract

Matrix metalloproteinases (MMPs) play numerous roles in physiological and pathological processes including cancer. Interactions of MMPs with other zinc-binding proteins are of great interest mainly from the point of view of the participation of these proteins in carcinogenesis. The aim of this study was to utilize chip gel electrophoresis for investigation of matrix metalloproteinase 9 (gelatinase B, MMP-9) interactions with collagen. The interaction was observed and the effect of temperature was tested. We further focused on the study of activation of MMP-9 by the presence of zinc-binding proteins called metallothioneins (MT). We confirmed the basic presumption of the activation of MMP-9 by the presence of MT similar to the temperature effect. Moreover, we chose the method of differential pulse voltammetry Brdicka reaction to verify our results. It can be concluded that the activity of MMP-9 was higher in the presence of MT in comparison with the experimental collagen(type I)-MMP-9 mixture in vitro.

Keywords

matrix metalloproteinases, carcinogenesis, cancer

Key words in English

matrix metalloproteinases, carcinogenesis, cancer

Authors

ZÍTKA, O.; HUBÁLEK, J.; KŘÍŽKOVÁ, S.; HÚSKA, D.; ADAM, V.; ECKSCHLAGER, T.; KIZEK, R.

RIV year

2013

Released

01.09.2011

ISBN

0173-0835

Periodical

ELECTROPHORESIS

Volume

2011 (32)

Number

8

State

Federal Republic of Germany

Pages from

857

Pages to

860

Pages count

4

BibTex

@article{BUT74828,
  author="ZÍTKA, O. and HUBÁLEK, J. and KŘÍŽKOVÁ, S. and HÚSKA, D. and ADAM, V. and ECKSCHLAGER, T. and KIZEK, R.",
  title="Chip gel electrophoresis as a tool for study of matrix metalloproteinase 9 interaction with metallothionein",
  journal="ELECTROPHORESIS",
  year="2011",
  volume="2011 (32)",
  number="8",
  pages="857--860",
  issn="0173-0835"
}