The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

Abstract

The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.

The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.

crude lyzates, Lactobacillus, isolation, DNA, PCR

crude lyzates, Lactobacillus, isolation, DNA, PCR

ČUTA, R.; FRIČOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.

2012

17.08.2011

Česká společnost chemická

Praha

0009-2770

CHEMICKE LISTY

105

18

Czech Republic

s1009

s1009

1