Monitoring of Yeasts Population in Ecological and Integrated Produced Moravian wine

Monitoring of Yeasts Population in Ecological and Integrated Produced Moravian wine

Abstract

In this study, we identified yeasts isolated from red variety Rulandske modre from South Moravia. Yeasts were isolated from must during different stages of fermentation process. We have chosen two types of vineyard agriculture ecological and integrated. In order to identify species we used PCR-RFLP method (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism). We amplified 5.8S ITS segment of rDNA by using ITS1 and ITS4 primers. Then amplified DNA was chopped by five restriction endonucleases HaeIII, HinfI, TaqI, AluI and MseI.

In this study, we identified yeasts isolated from red variety Rulandske modre from South Moravia. Yeasts were isolated from must during different stages of fermentation process. We have chosen two types of vineyard agriculture ecological and integrated. In order to identify species we used PCR-RFLP method (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism). We amplified 5.8S ITS segment of rDNA by using ITS1 and ITS4 primers. Then amplified DNA was chopped by five restriction endonucleases HaeIII, HinfI, TaqI, AluI and MseI.

yeasts, wine, PCR-RFLP

yeasts, wine, PCR-RFLP

ŠURANSKÁ, H.; VRÁNOVÁ, D.; JIŘÍKOVÁ, I.; PROCHÁZKOVÁ, L.; VADKERTIOVÁ, R.; OMELKOVÁ, J.

2012

14.09.2011

Česká společnost chemická

Praha

Chemické listy

0009-2770

CHEMICKE LISTY

105

18

Czech Republic

s1007

s1007

1