Publication detail

Monitoring of Yeasts Population in Ecological and Integrated Produced Moravian wine

ŠURANSKÁ, H. VRÁNOVÁ, D. JIŘÍKOVÁ, I. PROCHÁZKOVÁ, L. VADKERTIOVÁ, R. OMELKOVÁ, J.

Original Title

Monitoring of Yeasts Population in Ecological and Integrated Produced Moravian wine

Type

abstract

Language

English

Original Abstract

In this study, we identified yeasts isolated from red variety Rulandske modre from South Moravia. Yeasts were isolated from must during different stages of fermentation process. We have chosen two types of vineyard agriculture ecological and integrated. In order to identify species we used PCR-RFLP method (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism). We amplified 5.8S ITS segment of rDNA by using ITS1 and ITS4 primers. Then amplified DNA was chopped by five restriction endonucleases HaeIII, HinfI, TaqI, AluI and MseI.

Keywords

yeasts, wine, PCR-RFLP

Authors

ŠURANSKÁ, H.; VRÁNOVÁ, D.; JIŘÍKOVÁ, I.; PROCHÁZKOVÁ, L.; VADKERTIOVÁ, R.; OMELKOVÁ, J.

Released

14. 9. 2011

Publisher

Česká společnost chemická

Location

Praha

ISBN

0009-2770

Periodical

Chemické listy

Year of study

105

Number

18

State

Czech Republic

Pages from

s1007

Pages to

s1007

Pages count

1