Publication detail

Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces

TRACHTOVA, S. OBERMAJER, T. SPANOVA, A. BOGOVIC MATIJASIC, B. ROGELJ, I. HORAK, D. RITTICH, B.

Original Title

Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces

Type

journal article in Web of Science

Language

English

Original Abstract

Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of target DNA were verified by PCR and real time PCR using primers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxyl-coated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR.

Keywords

magnetic microspheres; c); DNA isolation; mouse faeces; polymerase chain reaction (PCR)

Authors

TRACHTOVA, S.; OBERMAJER, T.; SPANOVA, A.; BOGOVIC MATIJASIC, B.; ROGELJ, I.; HORAK, D.; RITTICH, B.

RIV year

2012

Released

15. 2. 2012

Publisher

Taylor & Francis

Location

Londýn, Spojené království Velké Británie a Severního Irska

ISBN

1542-1406

Periodical

MOLECULAR CRYSTALS AND LIQUID CRYSTALS

Year of study

555

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

263

Pages to

270

Pages count

7

BibTex

@article{BUT73651,
  author="Štěpánka {Trachtová} and Tanja {Obermajer} and Alena {Španová} and Bojana {Bogovič Matijašić} and Irena {Rogelj} and Daniel {Horák} and Bohuslav {Rittich}",
  title="Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces",
  journal="MOLECULAR CRYSTALS AND LIQUID CRYSTALS",
  year="2012",
  volume="555",
  number="1",
  pages="263--270",
  doi="10.1080/15421406.2012.635554",
  issn="1542-1406"
}