Characterization of magnetic and non-magnetic solid polymer microspheres

Characterization of magnetic and non-magnetic solid polymer microspheres

Abstract

Magnetic separation is one of modern methods to speed up and facilitate the previously used separation and purification procedures. The aim of this work was to study the potential inhibition effect of different types of commercial and non-commercial magnetic and non-magnetic carriers on PCR course. It was shown that most of carriers does not influence the course of PCR. In second part, reversible DNA adsorption on surface of magnetic particles was tested in the presence of an aqueous phase system containing 16 % PEG 6000 and 2 M NaCl from complex samples, namely dairy products.

Magnetic separation is one of modern methods to speed up and facilitate the previously used separation and purification procedures. The aim of this work was to study the potential inhibition effect of different types of commercial and non-commercial magnetic and non-magnetic carriers on PCR course. It was shown that most of carriers does not influence the course of PCR. In second part, reversible DNA adsorption on surface of magnetic particles was tested in the presence of an aqueous phase system containing 16 % PEG 6000 and 2 M NaCl from complex samples, namely dairy products.

magnetic- and non-magnetic particles, PCR, real time PCR, PCR inhibiton, dairy products

magnetic- and non-magnetic particles, PCR, real time PCR, PCR inhibiton, dairy products

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.; HORÁK, D.

2012

17.08.2011

česká společnost chemická

Praha, ČR

0009-2770

CHEMICKE LISTY

105

18

Czech Republic

s988

s989

2