Publication result detail

Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

Original Title

Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

English Title

Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

Type

Abstract

Original Abstract

The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.

English abstract

The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.

Keywords

ethidium monoazide; dead and live bacterial cells

Key words in English

ethidium monoazide; dead and live bacterial cells

Authors

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

Released

04.06.2008

Publisher

Univerzita P.J. Šafárika

Location

Košice, Slovenská Republika

Book

Proceedings o International Probiotic Conference, ISSN 1336-863X

Pages from

55

Pages to

55

Pages count

1

BibTex

@misc{BUT61084,
  author="Štěpánka {Trachtová} and Alena {Španová} and Bohuslav {Rittich}",
  title="Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells",
  booktitle="Proceedings o International Probiotic Conference, ISSN 1336-863X",
  year="2008",
  edition="1",
  pages="55--55",
  publisher="Univerzita P.J. Šafárika",
  address="Košice,
Slovenská Republika",
  note="Abstract"
}