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STEJSKAL, K.; KŘÍŽKOVÁ, S.; ADAM, V.; SURES, B.; TRNKOVÁ, L.; ZEHNÁLEK, J.; HUBÁLEK, J.; BEKLOVÁ, M.; HANUSTIAK, P.; SVOBODOVÁ, Z.; HORNA, A.; KIZEK, R.
Original Title
Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection
English Title
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, "current R": 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled with differential pulse voltammetry Brdicka reaction, which was used as the reference technique.
English abstract
Keywords
Amperometric detection; biomarker; electrochemical sensors; fish; flow injection analysis; heavy metal; metallothionein; poecilia reticulata; silver
Key words in English
Authors
RIV year
2013
Released
01.10.2008
Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Location
USA
ISBN
1530-437X
Periodical
IEEE SENSORS JOURNAL
Volume
8
Number
9-10
State
United States of America
Pages from
1578
Pages to
1585
Pages count
BibTex
@article{BUT47213, author="STEJSKAL, K. and KŘÍŽKOVÁ, S. and ADAM, V. and SURES, B. and TRNKOVÁ, L. and ZEHNÁLEK, J. and HUBÁLEK, J. and BEKLOVÁ, M. and HANUSTIAK, P. and SVOBODOVÁ, Z. and HORNA, A. and KIZEK, R.", title="Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection", journal="IEEE SENSORS JOURNAL", year="2008", volume="8", number="9-10", pages="1578--1585", issn="1530-437X" }