Holographic Confocal Microscopy

Holographic Confocal Microscopy

Paper in proceedings (conference paper)

Conventional confocal microscopes form the confocal image using various types of scanning systems. Besides other consequences of this fact, their maximum achievable imaging speed is limited. We report on the holographic confocal microscope which is based on a broad-spource image-plane holographic method adapted for a real-time reflected-light microscopy of the specimen surface regions. It is verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed which is not limited by any part of the optical system and thus can be much higher.

Conventional confocal microscopes form the confocal image using various types of scanning systems. Besides other consequences of this fact, their maximum achievable imaging speed is limited. We report on the holographic confocal microscope which is based on a broad-spource image-plane holographic method adapted for a real-time reflected-light microscopy of the specimen surface regions. It is verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed which is not limited by any part of the optical system and thus can be much higher.

confocal microscopy, holographic techniques

confocal microscopy, holographic techniques

CHMELÍK, R., HARNA, Z., MATOUŠEK, M.

01.01.1999

EMBL, Heidelberg, Germany

Heidelberg

Focus on Microscopy 1999

93

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