Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Paper in proceedings (conference paper)

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

digital holographic microscopy, phase image, deformation compensation, least square method

digital holographic microscopy, phase image, deformation compensation, least square method

ZIKMUND, T.; KVASNICA, L.; LOVICAR, L.; UHLÍŘOVÁ, H.; CHMELÍK, R.

2011

14.12.2010

SPIE

9780819482365

Proceedings of SPIE, Volume 7746

0277-786X

Proceedings of SPIE

United States of America

77460K-1

77460K-7

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