., Antimycotic effects of the plasma gun on C. glabrata on different surface materials

., Antimycotic effects of the plasma gun on C. glabrata on different surface materials

Abstract

This work studies an alternative treatment of otitis externa or so-called swimmer´s ear is a condition that causes inflammation of the external ear canal, which is the tube between the outer ear and the ear drum. The disease can develop in all mammalian species but due to several different factors the dogs are the main patients for the treatment. Due to the lack of efficient treatment methods for this multifactorial disease, the veterinary doctors are striving for a new alternative treatment. In this study, the cold atmospheric pressure plasma (CAP) generated by the plasma gun with a capillary design was tested. The use of the plasma gun with a thin capillary allows for an endoscopic treatment inside the complex structures. It was found that the thickness of a capillary does not influence the inhibition efficiency, when the plasma parameters are adjusted accordingly. The plasma inhibition is stable in time and the microbial cells do not regain their viability even after one week. It was also proved that using a cover (enclosed treatment) tremendously enhances the treatment efficiency. This fact is mainly caused by the accumulation of the active species (reactive oxygen and nitrogen species - RONS) under the cover. A generation of the RONS is highly dependent on the target, which is why several different inoculation substrates were tested. The results confirm high inhibition efficiency of CAP against the yeast C. glabrata on different surface materials.

This work studies an alternative treatment of otitis externa or so-called swimmer´s ear is a condition that causes inflammation of the external ear canal, which is the tube between the outer ear and the ear drum. The disease can develop in all mammalian species but due to several different factors the dogs are the main patients for the treatment. Due to the lack of efficient treatment methods for this multifactorial disease, the veterinary doctors are striving for a new alternative treatment. In this study, the cold atmospheric pressure plasma (CAP) generated by the plasma gun with a capillary design was tested. The use of the plasma gun with a thin capillary allows for an endoscopic treatment inside the complex structures. It was found that the thickness of a capillary does not influence the inhibition efficiency, when the plasma parameters are adjusted accordingly. The plasma inhibition is stable in time and the microbial cells do not regain their viability even after one week. It was also proved that using a cover (enclosed treatment) tremendously enhances the treatment efficiency. This fact is mainly caused by the accumulation of the active species (reactive oxygen and nitrogen species - RONS) under the cover. A generation of the RONS is highly dependent on the target, which is why several different inoculation substrates were tested. The results confirm high inhibition efficiency of CAP against the yeast C. glabrata on different surface materials.

antimicrobial effects, Candida glabrata, CAP, cold plasma, direct treatment

antimicrobial effects, Candida glabrata, CAP, cold plasma, direct treatment

TREBULOVÁ, K.; OREL, I.; ROBERT, E.; HRUDKA, J.; KRČMA, F.

30.11.2023

FCH VUT

Brno

Chemie je život, sborník abstraktů

72

72

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