Publication result detail

In vitro culture platform based on electroconductive BSA/PEDOT:PSS hydrogel

FOHLEROVÁ, Z.; POLÁKOVÁ, V.

Original Title

In vitro culture platform based on electroconductive BSA/PEDOT:PSS hydrogel

English Title

In vitro culture platform based on electroconductive BSA/PEDOT:PSS hydrogel

Type

Abstract

Original Abstract

The development of conductive hydrogel-based platforms combined with optics for studying cardiomyocytes and their interactions in vitro is of a great attention. Complementing the use of these platforms with computer modeling represents an innovative and unique approach for studying cardiac cellular electrophysiology and pathophysiology. The conductive hydrogel is prepared from natural bovine serum albumin (BSA) and conducting PEDOT:PSS polymer. The work involves preparation of hydrogels with desired mechanico-electrical properties, physico-chemical characterization of hydrogel and biocompatibility to cardiac cells. The preliminary results shows that BSA/PEDOT:PSS creates the optically transparent hydrogel with Young modulus and ionic conductivity about 20 kPa and 10-4 S·m, respectively. The hydrogel als shows good adhesion and non-toxicity to cardiac HL-1 cells. The proposed novel in vitro culture platforms can be used to study phenomena previously undiscoverable with electrically insulating cell culture substrates.

English abstract

The development of conductive hydrogel-based platforms combined with optics for studying cardiomyocytes and their interactions in vitro is of a great attention. Complementing the use of these platforms with computer modeling represents an innovative and unique approach for studying cardiac cellular electrophysiology and pathophysiology. The conductive hydrogel is prepared from natural bovine serum albumin (BSA) and conducting PEDOT:PSS polymer. The work involves preparation of hydrogels with desired mechanico-electrical properties, physico-chemical characterization of hydrogel and biocompatibility to cardiac cells. The preliminary results shows that BSA/PEDOT:PSS creates the optically transparent hydrogel with Young modulus and ionic conductivity about 20 kPa and 10-4 S·m, respectively. The hydrogel als shows good adhesion and non-toxicity to cardiac HL-1 cells. The proposed novel in vitro culture platforms can be used to study phenomena previously undiscoverable with electrically insulating cell culture substrates.

Keywords

condictive hydrogel, in vitro testing

Key words in English

condictive hydrogel, in vitro testing

Authors

FOHLEROVÁ, Z.; POLÁKOVÁ, V.

Released

02.07.2024

Location

Greece, Thessaloniki

Book

21st International Conference on Nanosciences & Nanotechnologies, Book of Abstract, Greece, Thessaloniki, 2-5 July 2024

Pages from

162

Pages to

162

Pages count

175

URL

https://www.nanotexnology.com/index.php/abstract-books-2024/send/5-abstract-books-2024/16-nn24-abstract-book

BibTex

@misc{BUT189721,
  author="Zdenka {Fohlerová} and Veronika {Poláková}",
  title="In vitro culture platform based on electroconductive BSA/PEDOT:PSS hydrogel",
  booktitle="21st International Conference on Nanosciences & Nanotechnologies, Book of Abstract, Greece, Thessaloniki, 2-5 July 2024",
  year="2024",
  pages="162--162",
  address="Greece, Thessaloniki",
  url="https://www.nanotexnology.com/index.php/abstract-books-2024/send/5-abstract-books-2024/16-nn24-abstract-book",
  note="Abstract"
}