Publication result detail

The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis

MACHELOVÁ, A.; DADEJOVÁ, M.; FRANEK, M.; MOUGEOT, G.; SIMON, L.; LE GOFF, S.; DUC, C.; BASSLER, J.; DEMKO, M.; SCHWARZEROVÁ, J.; DESSET, S.; PROBST, A.; DVOŘÁČKOVÁ, M.

Original Title

The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis

English Title

The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis

Type

WoS Article

Original Abstract

Genome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In this study, we investigate the role of the Arabidopsis H3 histone chaperones ANTI-SILENCING FUNCTION 1 (ASF1) and HISTONE REGULATOR A (HIRA) in the maintenance of telomeres and 45S rDNA loci, genomic sites that are particularly susceptible to changes in the chromatin structure. We find that both ASF1 and HIRA are essential for telomere length regulation, as telomeres are significantly shorter in asf1a1b and hira mutants. However, these shorter telomeres remain localized around the nucleolus and exhibit a comparable relative H3 occupancy to the wild type. In addition to regulating telomere length, ASF1 and HIRA contribute to silencing 45S rRNA genes and affect their copy number. Besides, ASF1 supports global heterochromatin maintenance. Our findings also indicate that ASF1 transiently binds to the TELOMERE REPEAT BINDING 1 protein and the N terminus of telomerase in vivo, suggesting a physical link between the ASF1 histone chaperone and the telomere maintenance machinery.

English abstract

Genome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In this study, we investigate the role of the Arabidopsis H3 histone chaperones ANTI-SILENCING FUNCTION 1 (ASF1) and HISTONE REGULATOR A (HIRA) in the maintenance of telomeres and 45S rDNA loci, genomic sites that are particularly susceptible to changes in the chromatin structure. We find that both ASF1 and HIRA are essential for telomere length regulation, as telomeres are significantly shorter in asf1a1b and hira mutants. However, these shorter telomeres remain localized around the nucleolus and exhibit a comparable relative H3 occupancy to the wild type. In addition to regulating telomere length, ASF1 and HIRA contribute to silencing 45S rRNA genes and affect their copy number. Besides, ASF1 supports global heterochromatin maintenance. Our findings also indicate that ASF1 transiently binds to the TELOMERE REPEAT BINDING 1 protein and the N terminus of telomerase in vivo, suggesting a physical link between the ASF1 histone chaperone and the telomere maintenance machinery.

Keywords

Flavobacterium psychrophilum, Genetic diversity, Antimicrobial resistance

Key words in English

Flavobacterium psychrophilum, Genetic diversity, Antimicrobial resistance

Authors

MACHELOVÁ, A.; DADEJOVÁ, M.; FRANEK, M.; MOUGEOT, G.; SIMON, L.; LE GOFF, S.; DUC, C.; BASSLER, J.; DEMKO, M.; SCHWARZEROVÁ, J.; DESSET, S.; PROBST, A.; DVOŘÁČKOVÁ, M.

RIV year

2025

Released

14.10.2024

Publisher

Wiley

ISBN

0960-7412

Periodical

PLANT JOURNAL

Volume

120

Number

3

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1125

Pages to

1141

Pages count

17

URL

https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.17041

Full text in the Digital Library

http://hdl.handle.net/11012/250729

BibTex

@article{BUT189692,
  author="Adéla {Machelová} and Martina {Dadejová} and Michal {Franek} and Guillaume {Mougeot} and Lauriane {Simon} and Samuel {Le Goff} and Celine {Duc} and Jasmin {Bassler} and Martin {Demko} and Jana {Schwarzerová} and Sophia {Desset} and Aline {Probst} and Martina {Dvořáčková}",
  title="The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis",
  journal="PLANT JOURNAL",
  year="2024",
  volume="120",
  number="3",
  pages="1125--1141",
  doi="10.1111/tpj.17041",
  issn="0960-7412",
  url="https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.17041"
}

Documents

2024-Machelova