Publication result detail

Quantitative analysis of Scratch assay and Colony formation assay data using MATLAB

INGROVÁ, K.; CHMELÍKOVÁ, L.; ZUMBERG, I.

Original Title

Quantitative analysis of Scratch assay and Colony formation assay data using MATLAB

English Title

Quantitative analysis of Scratch assay and Colony formation assay data using MATLAB

Type

Paper in proceedings (conference paper)

Original Abstract

Utilizing Scratch and Colony formation assays is crucial for understanding cell migration and proliferation dynamics. In this study, we present novel approaches utilizing MATLAB for the processing of images obtained from these assays. We demonstrate the effectiveness of our approaches through validation experiments on human osteosarcoma SaOS-2 cells, showcasing their applicability across different experimental conditions. Moreover, the inhibitory effect of two concentrations of gentamicin (0.1 mg/ml, 0.3 mg/ml) on the migration and clonogenicity of SaOS-2 cells was declared.

English abstract

Utilizing Scratch and Colony formation assays is crucial for understanding cell migration and proliferation dynamics. In this study, we present novel approaches utilizing MATLAB for the processing of images obtained from these assays. We demonstrate the effectiveness of our approaches through validation experiments on human osteosarcoma SaOS-2 cells, showcasing their applicability across different experimental conditions. Moreover, the inhibitory effect of two concentrations of gentamicin (0.1 mg/ml, 0.3 mg/ml) on the migration and clonogenicity of SaOS-2 cells was declared.

Keywords

Gentamicin, SaOS-2, Scratch assay, Colony formation assay, MATLAB

Key words in English

Gentamicin, SaOS-2, Scratch assay, Colony formation assay, MATLAB

Authors

INGROVÁ, K.; CHMELÍKOVÁ, L.; ZUMBERG, I.

RIV year

2025

Released

23.04.2024

Publisher

Brno University of Technology, Faculty of Electrical Engineering and Communication

Location

Brno

ISBN

978-80-214-6230-4

Book

Proceedings II of the 30th Conference STUDENT EEICT 2024 Selected Papers

ISBN

2788-1334

Periodical

Proceedings II of the Conference STUDENT EEICT

State

Czech Republic

Pages from

61

Pages to

64

Pages count

4

URL

https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf

BibTex

@inproceedings{BUT188885,
  author="Kateřina {Ingrová} and Larisa {Chmelíková} and Inna {Zumberg}",
  title="Quantitative analysis of Scratch assay and Colony formation assay data using MATLAB",
  booktitle="Proceedings II of the 30th Conference STUDENT EEICT 2024 Selected Papers",
  year="2024",
  journal="Proceedings II of the Conference STUDENT EEICT",
  number="1",
  pages="61--64",
  publisher="Brno University of Technology, Faculty of Electrical Engineering and Communication",
  address="Brno",
  doi="10.13164/eeict.2024.61",
  isbn="978-80-214-6230-4",
  url="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf"
}