Publication result detail

Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds

OLIVER URRUTIA, C.; DIEZ-ESCUDERO, A.; KOLEDOVÁ, Z.; ČELKO, L.; HAILER, N. P.; MONTUFAR JIMENEZ, E.

Original Title

Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds

English Title

Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds

Type

Chapter in a book

Original Abstract

In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds and bone implants. In this chapter, a protocol for a static three-dimensional osteoblast cell culture on titanium scaffolds and subsequent analysis of osteogenic capacity is presented. The protocol is explained for additively manufactured titanium scaffolds, but it can be extrapolated to other scaffolds with similar size and structure, while differing in composition or manufactured technology. Additionally, the protocol can be used for culture of other adherent cell types beyond osteoblast cells such as mesenchymal stem cells.

English abstract

In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds and bone implants. In this chapter, a protocol for a static three-dimensional osteoblast cell culture on titanium scaffolds and subsequent analysis of osteogenic capacity is presented. The protocol is explained for additively manufactured titanium scaffolds, but it can be extrapolated to other scaffolds with similar size and structure, while differing in composition or manufactured technology. Additionally, the protocol can be used for culture of other adherent cell types beyond osteoblast cells such as mesenchymal stem cells.

Keywords

cell cultures; In vitro; cytocompatibility; titanium scaffolds

Key words in English

cell cultures; In vitro; cytocompatibility; titanium scaffolds

Authors

OLIVER URRUTIA, C.; DIEZ-ESCUDERO, A.; KOLEDOVÁ, Z.; ČELKO, L.; HAILER, N. P.; MONTUFAR JIMENEZ, E.

RIV year

2025

Released

24.02.2024

Publisher

Humana

Location

New York, NY

ISBN

978-1-0716-3673-2

Book

Methods in Molecular Biology

Edition

2764

Pages from

43

Pages to

60

Pages count

17

URL

https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_5#citeas

BibTex

@inbook{BUT188701,
  author="OLIVER URRUTIA, C. and DIEZ-ESCUDERO, A. and KOLEDOVÁ, Z. and ČELKO, L. and HAILER, N. P. and MONTUFAR JIMENEZ, E.",
  title="Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds",
  booktitle="Methods in Molecular Biology",
  year="2024",
  publisher="Humana",
  address="New York, NY",
  series="2764",
  pages="43--60",
  doi="10.1007/978-1-0716-3674-9\{_}5",
  isbn="978-1-0716-3673-2",
  url="https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_5#citeas"
}