Publication result detail

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

ZHANG, H.; CAO, L.; BRODSKÝ, J.; GABLECH, I.; XU, F.; LI, Z.; KORABEČNÁ, M.; NEUŽIL, P.

Original Title

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

English Title

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

Type

WoS Article

Original Abstract

This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR (dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from pathogen detection, and cancer screening to environment monitoring. The manuscript outlines the foundational theories behind both technologies and scrutinizes their capabilities, advances, and limits. It deeply investigates the primary factors based on the sample's nature, sample preparation strategies, and employing key parameters like reliability, accuracy, and specificity, thus critically evaluating the merits and demerits of each technology. It also proposes the avenues of research and development that impact both technologies. The paper concludes that the choice between qPCR and dPCR depends on factors such as the nature of the sample, the purpose of the study, and practical considerations like cost and operational ease. Our overarching goal is to furnish a guide that aids researchers in selecting the optimal PCR-based technique for their assay development.

English abstract

This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR (dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from pathogen detection, and cancer screening to environment monitoring. The manuscript outlines the foundational theories behind both technologies and scrutinizes their capabilities, advances, and limits. It deeply investigates the primary factors based on the sample's nature, sample preparation strategies, and employing key parameters like reliability, accuracy, and specificity, thus critically evaluating the merits and demerits of each technology. It also proposes the avenues of research and development that impact both technologies. The paper concludes that the choice between qPCR and dPCR depends on factors such as the nature of the sample, the purpose of the study, and practical considerations like cost and operational ease. Our overarching goal is to furnish a guide that aids researchers in selecting the optimal PCR-based technique for their assay development.

Keywords

qPCR; dPCR

Key words in English

qPCR; dPCR

Authors

ZHANG, H.; CAO, L.; BRODSKÝ, J.; GABLECH, I.; XU, F.; LI, Z.; KORABEČNÁ, M.; NEUŽIL, P.

RIV year

2025

Released

01.05.2024

Publisher

Elsevier

Location

UK

ISBN

0165-9936

Periodical

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Volume

174

Number

5

State

United Kingdom of Great Britain and Northern Ireland

Pages count

17

URL

https://www.sciencedirect.com/science/article/pii/S0165993624001584

BibTex

@article{BUT188407,
  author="ZHANG, H. and CAO, L. and BRODSKÝ, J. and GABLECH, I. and XU, F. and LI, Z. and KORABEČNÁ, M. and NEUŽIL, P.",
  title="Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications",
  journal="TRAC-TRENDS IN ANALYTICAL CHEMISTRY",
  year="2024",
  volume="174",
  number="5",
  pages="17",
  doi="10.1016/j.trac.2024.117676",
  issn="0165-9936",
  url="https://www.sciencedirect.com/science/article/pii/S0165993624001584"
}