Publication detail

Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems

MIŠÍK, O. SZABOVÁ, J. CEJPEK, O. MALÝ, M. JUGL, A. BĚLKA, M. MRAVEC, F. LÍZAL, F.

Original Title

Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems

Type

journal article in Web of Science

Language

English

Original Abstract

Liposomal carrier systems have emerged as a promising technology for pulmonary drug delivery. This study focuses on two selected liposomal systems, namely, dipalmitoylphosphatidylcholine stabilized by phosphatidic acid and cholesterol (DPPC-PA-Chol) and dipalmitoylphosphatidylcholine stabilized by polyethylene glycol and cholesterol (DPPC-PEG-Chol). First, the research investigates the stability of these liposomal systems during the atomization process using different kinds of nebulizers (air-jet, vibrating mesh, and ultrasonic). The study further explores the aerodynamic particle size distribution of the aerosol generated by the nebulizers. The nebulizer that demonstrated optimal stability and particle size was selected for more detailed investigation, including Andersen cascade impactor measurements, an assessment of the influence of flow rate and breathing profiles on aerosol particle size, and an in vitro deposition study on a realistic replica of the upper airways. The most suitable combination of a nebulizer and liposomal system was DPPC-PA-Chol nebulized by a Pari LC Sprint Star in terms of stability and particle size. The influence of the inspiration flow rate on the particle size was not very strong but was not negligible either (decrease of D-v50 by 1.34 mu m with the flow rate increase from 8 to 60 L/min). A similar effect was observed for realistic transient inhalation. According to the in vitro deposition measurement, approximately 90% and 70% of the aerosol penetrated downstream of the trachea using the stationary flow rate and the realistic breathing profile, respectively. These data provide an image of the potential applicability of liposomal carrier systems for nebulizer therapy. Regional lung drug deposition is patient-specific; therefore, deposition results might vary for different airway geometries. However, deposition measurement with realistic boundary conditions (airway geometry, breathing profile) brings a more realistic image of the drug delivery by the selected technology. Our results show how much data from cascade impactor testing or estimates from the fine fraction concept differ from those of a more realistic case.

Keywords

liposome; aerosol; particle size; nebulizer; pulmonary drug delivery; inhalation; deposition

Authors

MIŠÍK, O.; SZABOVÁ, J.; CEJPEK, O.; MALÝ, M.; JUGL, A.; BĚLKA, M.; MRAVEC, F.; LÍZAL, F.

Released

11. 3. 2024

Publisher

American Chemical Society

Location

WASHINGTON

ISBN

1543-8384

Periodical

MOLECULAR PHARMACEUTICS

Year of study

21

Number

4

State

United States of America

Pages from

1848

Pages to

1860

Pages count

13

URL

https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.3c01146

Full text in the Digital Library

http://hdl.handle.net/11012/245516

BibTex

@article{BUT188404,
  author="Ondrej {Mišík} and Jana {Szabová} and Ondřej {Cejpek} and Milan {Malý} and Adam {Jugl} and Miloslav {Bělka} and Filip {Mravec} and František {Lízal}",
  title="Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems",
  journal="MOLECULAR PHARMACEUTICS",
  year="2024",
  volume="21",
  number="4",
  pages="13",
  doi="10.1021/acs.molpharmaceut.3c01146",
  issn="1543-8384",
  url="https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.3c01146"
}