Publication result detail

Smallest dual-color qPCR device

Hanliang Zhu; Xiaocheng Liu; Yingqi Wang; Antao Sun; Tomas Teply; Marie Korabecna; Haoqing Zhang; Pavel Neuzil

Original Title

Smallest dual-color qPCR device

English Title

Smallest dual-color qPCR device

Type

WoS Article

Original Abstract

The global outbreak of the coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), necessitated the development of point-of-care (POC) systems for rapid and widespread population screening. Current POC systems, based on reverse transcription–quantitative polymerase chain reaction (RT-qPCR) methods, are unable to perform RNA target multiplexing, which is crucial for specific viral strain identification. This study presents a compact qPCR system capable of dual fluorescence channel gene multiplexing by simultaneously detecting two targets of the SARS-CoV-2 virus using 6-carboxyfluorescein (FAM)-and TxRed-labeled probes. The device measures (110 × 83 × 41) mm3 and weighs 210 gm. It features four virtual reaction chambers, a thermal cycle system, a real-time optical signal detection module, a USB interface, and a Bluetooth wireless data transmission module. Moreover, it can perform single fluorescent channel detection of PCR using intercalating dye, followed by a melting curve analysis and loop-mediated isothermal amplification. The proposed compact dual-color qPCR device significantly broadens its potential for POC applications, such as diagnostics or field testing, molecular biology, microfluidics, and virus detection.

English abstract

The global outbreak of the coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), necessitated the development of point-of-care (POC) systems for rapid and widespread population screening. Current POC systems, based on reverse transcription–quantitative polymerase chain reaction (RT-qPCR) methods, are unable to perform RNA target multiplexing, which is crucial for specific viral strain identification. This study presents a compact qPCR system capable of dual fluorescence channel gene multiplexing by simultaneously detecting two targets of the SARS-CoV-2 virus using 6-carboxyfluorescein (FAM)-and TxRed-labeled probes. The device measures (110 × 83 × 41) mm3 and weighs 210 gm. It features four virtual reaction chambers, a thermal cycle system, a real-time optical signal detection module, a USB interface, and a Bluetooth wireless data transmission module. Moreover, it can perform single fluorescent channel detection of PCR using intercalating dye, followed by a melting curve analysis and loop-mediated isothermal amplification. The proposed compact dual-color qPCR device significantly broadens its potential for POC applications, such as diagnostics or field testing, molecular biology, microfluidics, and virus detection.

Keywords

Miniaturization/integration; Multiplexing; RT-qPCR; Virus detection

Key words in English

Miniaturization/integration; Multiplexing; RT-qPCR; Virus detection

Authors

Hanliang Zhu; Xiaocheng Liu; Yingqi Wang; Antao Sun; Tomas Teply; Marie Korabecna; Haoqing Zhang; Pavel Neuzil

RIV year

2025

Released

01.11.2023

Publisher

ELSEVIER SCIENCE SA

Location

LAUSANNE

ISBN

0925-4005

Periodical

SENSORS AND ACTUATORS B-CHEMICAL

Volume

394

Number

November 2023

State

Swiss Confederation

Pages from

1

Pages to

9

Pages count

9

URL

https://www.sciencedirect.com/science/article/pii/S0925400523010146

BibTex

@article{BUT186765,
  author="Hanliang Zhu and Xiaocheng Liu and Yingqi Wang and Antao Sun and Tomas Teply and Marie Korabecna and Haoqing Zhang and Pavel Neuzil",
  title="Smallest dual-color qPCR device",
  journal="SENSORS AND ACTUATORS B-CHEMICAL",
  year="2023",
  volume="394",
  number="November 2023",
  pages="1--9",
  doi="10.1016/j.snb.2023.134299",
  url="https://www.sciencedirect.com/science/article/pii/S0925400523010146"
}