Publication detail

Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia

MATIAŠOVIC, J. BZDIL, J. PAPEŽÍKOVÁ, I. ČEJKOVÁ, D. VASINA, E. BIZOS, J. NAVRÁTIL, S. ŠEDIVÁ, M. KLAUDINY, J. PIKULA, J.

Original Title

Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia

Type

journal article in Web of Science

Language

English

Original Abstract

Paenibacillus larvae is the causative agent of American foulbrood (AFB), a devastating disease of honeybee larvae. In the Czech Republic, two large infested regions were recognised. This study aimed to analyse P. larvae strains occurring in the Czech Republic in the years 2016–2017 and to characterise the genetic structure of their population with the use of Enterobacterial Repetitive Intergenic Consensus genotyping (ERIC), multilocus sequence typing (MLST) and whole genome sequence (WGS) analysis. The results were complemented by the analysis of isolates collected in the year 2018 in areas of Slovakia located near the Czechia-Slovakia border. ERIC genotyping revealed that 78.9% of tested isolates belonged to the ERIC II genotype and 21.1% to ERIC I genotype. MLST showed six sequence types with ST10 and ST11 being the most frequent among isolates. Within six isolates we found discrepancies in correlations between MLST and ERIC genotypes. The use of MLST and WGS analysis of isolates revealed that each of the large infested geographic regions had its own dominating P. larvae strains. We assume that these strains represented primary sources of infection in the affected areas. In addition, the sporadic presence of strains identified by core genome analysis as genetically related was unveiled in geographically distant regions suggesting possible human-mediated transmission of AFB.

Keywords

American foulbroodGenotypingEpidemiologyHoneybeeAFB spread

Authors

MATIAŠOVIC, J.; BZDIL, J.; PAPEŽÍKOVÁ, I.; ČEJKOVÁ, D.; VASINA, E.; BIZOS, J.; NAVRÁTIL, S.; ŠEDIVÁ, M.; KLAUDINY, J.; PIKULA, J.

Released

8. 3. 2023

Publisher

ELSEVIER

ISBN

1532-2661

Periodical

RESEARCH IN VETERINARY SCIENCE

Year of study

158

Number

3

State

United Kingdom of Great Britain and Northern Ireland

Pages from

34

Pages to

40

Pages count

7

URL

https://www.sciencedirect.com/science/article/pii/S0034528823000723?utm_campaign=STMJ_AUTH_SERV_PUBLISHED&utm_medium=email&utm_acid=267549703&SIS_ID=&dgcid=STMJ_AUTH_SERV_PUBLISHED&CMX_ID=&utm_in=DM348155&utm_source=AC_

BibTex

@article{BUT183134,
  author="Ján {Matiašovic} and Jaroslav {Bzdil} and Ivana {Papežíková} and Darina {Čejková} and Evgeniya {Vasina} and Jiří {Bizos} and Stanislav {Navrátil} and Mária {Šedivá} and Jaroslav {Klaudiny} and Jiří {Pikula}",
  title="Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia",
  journal="RESEARCH IN VETERINARY SCIENCE",
  year="2023",
  volume="158",
  number="3",
  pages="34--40",
  doi="10.1016/j.rvsc.2023.03.007",
  issn="1532-2661",
  url="https://www.sciencedirect.com/science/article/pii/S0034528823000723?utm_campaign=STMJ_AUTH_SERV_PUBLISHED&utm_medium=email&utm_acid=267549703&SIS_ID=&dgcid=STMJ_AUTH_SERV_PUBLISHED&CMX_ID=&utm_in=DM348155&utm_source=AC_"
}