Publication detail

Production of high-value added exopolysaccharide by biotherapeutic potential Lactobacillus reuteri strain

Dailin, D.J., Selvamani, S., Michelle, K., Jusoh, Y.M.M., Chuah, L.F., Bokhari, A., Enshasy, H.A.E., Mubashir, M., Show, P.L.

Original Title

Production of high-value added exopolysaccharide by biotherapeutic potential Lactobacillus reuteri strain

Type

journal article in Web of Science

Language

English

Original Abstract

Exopolysaccharides (EPS) are produced by various microbial groups including bacteria. EPS produced from Lactobacilli is gaining popularity as a new generation functional food to replace various chemically synthesized food additives. EPS production by strains of L. reuteri were reported previously in sourdough fermentation. The submerged cultivation of L. reuteri strain DSM 20016 T was optimised for crude EPS production. Initially, the factors controlling crude EPS generation were explored using one-at-a-time (OFAT). Among the investigated carbon sources, sucrose supplemented medium increased maximal crude EPS generation. Yeast extract was determined to be the greatest nitrogen source for increasing crude EPS and cell mass. Plackett-Burman experimental design (PBD) was employed to screen the important parameters impacting crude EPS production in the growing medium. Three important factors were identified: sucrose, yeast extract and sodium acetate. The RSM quadratic model for crude EPS generation was significant (R2:0.9373) and correlated with the observed response at roughly 94%. About 3.4 g L−1 crude EPS was produced under optimal fermentation conditions of sucrose (106.0 g L−1), yeast extract (30.0 g L−1) and sodium acetate (7.0 g L−1). This study shows the crude EPS synthesis of L. reuteri from sourdough can be used in submerged fermentation and biotechnological applications. © 2022 Elsevier B.V.

Keywords

Exopolysaccharides; Lactobacillus reuteri; Optimization; Response surface methodology; Submerged fermentation

Authors

Dailin, D.J., Selvamani, S., Michelle, K., Jusoh, Y.M.M., Chuah, L.F., Bokhari, A., Enshasy, H.A.E., Mubashir, M., Show, P.L.

Released

15. 12. 2022

Publisher

Elsevier

ISBN

1369-703X

Periodical

BIOCHEMICAL ENGINEERING JOURNAL

Number

188

State

Kingdom of the Netherlands

Pages count

10

URL

https://www.sciencedirect.com/science/article/pii/S1369703X22003606?via%3Dihub

BibTex

@article{BUT182768,
  author="Syed Awais Ali Shah {Bokhari}",
  title="Production of high-value added exopolysaccharide by biotherapeutic potential Lactobacillus reuteri strain",
  journal="BIOCHEMICAL ENGINEERING JOURNAL",
  year="2022",
  number="188",
  pages="10",
  doi="10.1016/j.bej.2022.108691",
  issn="1369-703X",
  url="https://www.sciencedirect.com/science/article/pii/S1369703X22003606?via%3Dihub"
}