Publication result detail

Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application

Li, Zhonghe; Gao, Xingai; Li, Ming; Yan, Qiuliang; Zhang, Nan; Yu, Boyang; Zhang, Bimi; Zhang, Shuying; Helal, Mohamed H.; Ali, Ola A. Abu; Nassan, Mohamed A.; Qyyum, Muhammad Abdul; Asif, Saira; Bokhari, Awais

Original Title

Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application

English Title

Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application

Type

WoS Article

Original Abstract

Steroid hormones as a class of emerging organic pollutant and high concern, due to their potential risks for human and environmental. Accurate analytical methods of steroid hormones are necessary in quantifying and monitoring. Biosensor is a promising technique. In this study, though part of 3 alpha-HSD DNA to construct a regulatory plasmid and with the EGFP reporter gene to generate a reporter plasmid. Separately transformed into Escherichia coli strain BL21 and extracted the cell lysates as novel biosensor reagents. Analyzed the total amounts of steroid hormones in water, sediment, and soil samples using biosensor reagents, and compared these results with those obtained by HPLC. In summary, detection method using an EGFP reporter that can detect trace amounts of steroid hormones to reached fg/L. The optimal reaction time range and temperature were 30 min and 30 degrees C, respectively, while the most suitable organic solvent for the steroid hormone was 100% ethanol, up to 96well plate format. This method is very suitable for high-throughput detection of environmental steroid hormone pollutants.

English abstract

Steroid hormones as a class of emerging organic pollutant and high concern, due to their potential risks for human and environmental. Accurate analytical methods of steroid hormones are necessary in quantifying and monitoring. Biosensor is a promising technique. In this study, though part of 3 alpha-HSD DNA to construct a regulatory plasmid and with the EGFP reporter gene to generate a reporter plasmid. Separately transformed into Escherichia coli strain BL21 and extracted the cell lysates as novel biosensor reagents. Analyzed the total amounts of steroid hormones in water, sediment, and soil samples using biosensor reagents, and compared these results with those obtained by HPLC. In summary, detection method using an EGFP reporter that can detect trace amounts of steroid hormones to reached fg/L. The optimal reaction time range and temperature were 30 min and 30 degrees C, respectively, while the most suitable organic solvent for the steroid hormone was 100% ethanol, up to 96well plate format. This method is very suitable for high-throughput detection of environmental steroid hormone pollutants.

Keywords

Fluorescent biosensor; Steroid hormone; EGFP-Tagged; Sediment; Soil; Water; BACTERIAL BIOSENSORS; DRINKING-WATER; ESTROGENS; BIOAVAILABILITY; CONSTRUCTION; EXTRACTION; SENSORS; RIVER; ASSAY

Key words in English

Fluorescent biosensor; Steroid hormone; EGFP-Tagged; Sediment; Soil; Water; BACTERIAL BIOSENSORS; DRINKING-WATER; ESTROGENS; BIOAVAILABILITY; CONSTRUCTION; EXTRACTION; SENSORS; RIVER; ASSAY

Authors

Li, Zhonghe; Gao, Xingai; Li, Ming; Yan, Qiuliang; Zhang, Nan; Yu, Boyang; Zhang, Bimi; Zhang, Shuying; Helal, Mohamed H.; Ali, Ola A. Abu; Nassan, Mohamed A.; Qyyum, Muhammad Abdul; Asif, Saira; Bokhari, Awais

RIV year

2023

Released

01.12.2022

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE

Location

San Diego

ISBN

0013-9351

Periodical

Environmental Research

Volume

2

Number

215

State

United States of America

Pages count

7

URL

https://www.sciencedirect.com/science/article/pii/S0013935122016309?via%3Dihub

BibTex

@article{BUT182642,
  author="Li, Zhonghe and Gao, Xingai and Li, Ming and Yan, Qiuliang and Zhang, Nan and Yu, Boyang and Zhang, Bimi and Zhang, Shuying and Helal, Mohamed H. and Ali, Ola A. Abu and Nassan, Mohamed A. and Qyyum, Muhammad Abdul and Asif, Saira and Bokhari, Awais",
  title="Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application",
  journal="Environmental Research",
  year="2022",
  volume="2",
  number="215",
  pages="7",
  doi="10.1016/j.envres.2022.114303",
  issn="0013-9351",
  url="https://www.sciencedirect.com/science/article/pii/S0013935122016309?via%3Dihub"
}