Publication detail

Next Generation of Bio-inoculants, their Preparation and Viability for the Agricultural Applications

ČERNAYOVÁ, D. SEDLÁČEK, P. SLANINOVÁ, E. TRUDIČOVÁ, M. OBRUČA, S.

Original Title

Next Generation of Bio-inoculants, their Preparation and Viability for the Agricultural Applications

Type

abstract

Language

English

Original Abstract

Azotobacter vinelandii is a plant growth-promoting rhizobacterium (PGPR), capable of synthesizing two different biopolymers with great application potential. Polyhydroxyalkanoates (PHAs) are accumulated in a form of intracellular granules, whereas alginate is produced extracellularly. In agricultural demands, the use of PGPRs as bio-inoculants increasing plant and crop yields can be an ecological alternative to conventional fertilizers. To protect bacterial cells and sustain long-term effect of inoculant application, we developed the novel concept in which bacteria are encapsulated into hydrogel-based carriers by using self-produced alginate. In this work, the bacterial culture was crosslinked with a gelation agent (calcium chloride solution) to form a hydrogel carrier with encapsulated bacteria. The produced alginate ranges between 1,67 – 4.9 g/l depending on the strain, whereas the content of PHA varies from 30 to 50% of cdw in all the strains. The stability and strength of the bacterial gels were compared by rheological analysis. Moreover, according to our results the bacteria entrapped in a gel matrix are viable and cultivatable, which confirms the feasibility and viability of the novel concept.

Keywords

Plant growth-promoting rhizobacteria, PGPRs, alginate, polyhydroxyalkanoates, PHAs, bioinoculants, encapsulation

Authors

ČERNAYOVÁ, D.; SEDLÁČEK, P.; SLANINOVÁ, E.; TRUDIČOVÁ, M.; OBRUČA, S.

Released

7. 10. 2022

Pages count

1

BibTex

@misc{BUT180813,
  author="Diana {Černayová} and Petr {Sedláček} and Eva {Slaninová} and Monika {Trudičová} and Stanislav {Obruča}",
  title="Next Generation of Bio-inoculants, their Preparation and Viability for the Agricultural Applications",
  year="2022",
  pages="1",
  note="abstract"
}