Publication result detail

Enzyme-Photocatalyst Tandem Microrobot Powered by Urea for Escherichia coli Biofilm Eradication

VILLA, K.; SOPHA, H.; ZELENKA, J.; MOTOLA, M.; DĚKANOVSKÝ, L.; CHYLII BEKETOVA, D.; MACÁK, J.; RUML, T.; PUMERA, M.

Original Title

Enzyme-Photocatalyst Tandem Microrobot Powered by Urea for Escherichia coli Biofilm Eradication

English Title

Enzyme-Photocatalyst Tandem Microrobot Powered by Urea for Escherichia coli Biofilm Eradication

Type

WoS Article

Original Abstract

Urinary-based infections affect millions of people worldwide. Such bacterial infections are mainly caused by Escherichia coli (E. coli) biofilm formation in the bladder and/or urinary catheters. Herein, the authors present a hybrid enzyme/photocatalytic microrobot, based on urease-immobilized TiO2/CdS nanotube bundles, that can swim in urea as a biocompatible fuel and respond to visible light. Upon illumination for 2 h, these microrobots are able to remove almost 90% of bacterial biofilm, due to the generation of reactive radicals, while bare TiO2/CdS photocatalysts (non-motile) or urease-coated microrobots in the dark do not show any toxic effect. These results indicate a synergistic effect between the self-propulsion provided by the enzyme and the photocatalytic activity induced under light stimuli. This work provides a photo-biocatalytic approach for the design of efficient light-driven microrobots with promising applications in microbiology and biomedicine.

English abstract

Urinary-based infections affect millions of people worldwide. Such bacterial infections are mainly caused by Escherichia coli (E. coli) biofilm formation in the bladder and/or urinary catheters. Herein, the authors present a hybrid enzyme/photocatalytic microrobot, based on urease-immobilized TiO2/CdS nanotube bundles, that can swim in urea as a biocompatible fuel and respond to visible light. Upon illumination for 2 h, these microrobots are able to remove almost 90% of bacterial biofilm, due to the generation of reactive radicals, while bare TiO2/CdS photocatalysts (non-motile) or urease-coated microrobots in the dark do not show any toxic effect. These results indicate a synergistic effect between the self-propulsion provided by the enzyme and the photocatalytic activity induced under light stimuli. This work provides a photo-biocatalytic approach for the design of efficient light-driven microrobots with promising applications in microbiology and biomedicine.

Keywords

bacterial biofilms; enzymatic; micromotors; photocatalysis; TiO; (2) nanotubes; urinary infections

Key words in English

bacterial biofilms; enzymatic; micromotors; photocatalysis; TiO; (2) nanotubes; urinary infections

Authors

VILLA, K.; SOPHA, H.; ZELENKA, J.; MOTOLA, M.; DĚKANOVSKÝ, L.; CHYLII BEKETOVA, D.; MACÁK, J.; RUML, T.; PUMERA, M.

RIV year

2023

Released

01.09.2022

Publisher

Wiley-VCH

Location

WEINHEIM

ISBN

1613-6829

Periodical

Small

Volume

18

Number

36

State

Federal Republic of Germany

Pages from

1

Pages to

8

Pages count

8

URL

https://onlinelibrary.wiley.com/doi/10.1002/smll.202106612

BibTex

@article{BUT178709,
  author="Katherine {Villa} and Hanna Ingrid {Sopha} and Jaroslav {Zelenka} and Martin {Motola} and Lukáš {Děkanovský} and Darya {Chylii Beketova} and Jan {Macák} and Tomáš {Ruml} and Martin {Pumera}",
  title="Enzyme-Photocatalyst Tandem Microrobot Powered by Urea for Escherichia coli Biofilm Eradication",
  journal="Small",
  year="2022",
  volume="18",
  number="36",
  pages="1--8",
  doi="10.1002/smll.202106612",
  issn="1613-6810",
  url="https://onlinelibrary.wiley.com/doi/10.1002/smll.202106612"
}