Publication detail

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

SEDLÁČEK, I. HOLOCHOVÁ, P. BUSSE, H. KOUBLOVÁ, V. KRÁLOVÁ, S. ŠVEC, P. SOBOTKA, R. STAŇKOVÁ, E. PILNÝ, J. ŠEDO, O. SMOLÍKOVÁ, J. SEDLÁŘ, K.

Original Title

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

Type

journal article in Web of Science

Language

English

Original Abstract

A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398(T) were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6-100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA-DNA hybridisation values clearly proved that P8398(T) represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398(T) (= CCM 8941(T) = LMG 32108(T)).

Keywords

Massilia; violacein; psychrophilic; description; whole-genome sequencing; Antarctica

Authors

SEDLÁČEK, I.; HOLOCHOVÁ, P.; BUSSE, H.; KOUBLOVÁ, V.; KRÁLOVÁ, S.; ŠVEC, P.; SOBOTKA, R.; STAŇKOVÁ, E.; PILNÝ, J.; ŠEDO, O.; SMOLÍKOVÁ, J.; SEDLÁŘ, K.

Released

24. 3. 2022

Publisher

MDPI

Location

BASEL

ISBN

2076-2607

Periodical

Microorganisms

Year of study

10

Number

4

State

Swiss Confederation

Pages from

1

Pages to

14

Pages count

14

URL

https://www.mdpi.com/2076-2607/10/4/704/htm

Full text in the Digital Library

http://hdl.handle.net/11012/204462

BibTex

@article{BUT177721,
  author="Ivo {Sedláček} and Pavla {Holochová} and Hans-Jürgen {Busse} and Vendula {Koublová} and Stanislava {Králová} and Pavel {Švec} and Roman {Sobotka} and Eva {Staňková} and Jan {Pilný} and Ondrej {Šedo} and Jana {Smolíková} and Karel {Sedlář}",
  title="Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.",
  journal="Microorganisms",
  year="2022",
  volume="10",
  number="4",
  pages="1--14",
  doi="10.3390/microorganisms10040704",
  issn="2076-2607",
  url="https://www.mdpi.com/2076-2607/10/4/704/htm"
}