Publication result detail

Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods

POMPEIANO VANÍČKOVÁ, L.; DO, T.; VEJVODOVÁ, M.; HORÁK, V.; HUBÁLEK, M.; EMRI, G.; ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; KŘÍŽKOVÁ, S.; FALTUSOVÁ, V.; POMPEIANO, A.; VACULOVIČOVÁ, M.; ZÍTKA, O.; VACULOVIČ, T.; ADAM, V.

Original Title

Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods

English Title

Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods

Type

WoS Article

Original Abstract

Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.

English abstract

Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.

Keywords

Melanoma; Mass spectrometry; MeLiM

Key words in English

Melanoma; Mass spectrometry; MeLiM

Authors

POMPEIANO VANÍČKOVÁ, L.; DO, T.; VEJVODOVÁ, M.; HORÁK, V.; HUBÁLEK, M.; EMRI, G.; ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; KŘÍŽKOVÁ, S.; FALTUSOVÁ, V.; POMPEIANO, A.; VACULOVIČOVÁ, M.; ZÍTKA, O.; VACULOVIČ, T.; ADAM, V.

RIV year

2022

Released

01.02.2022

ISBN

0141-8130

Periodical

International Journal of Biological Macromolecules

Volume

203

Number

1

State

Kingdom of the Netherlands

Pages from

583

Pages to

592

Pages count

10

URL

https://doi.org/10.1016/j.ijbiomac.2022.01.139

BibTex

@article{BUT176883,
  author="Lucie {Pompeiano Vaníčková} and Tomáš {Do} and Markéta {Vejvodová} and Vratislav {Horák} and Martin {Hubálek} and Gabriela {Emri} and Kristýna {Zemánková} and Kristýna {Pavelicová} and Soňa {Křížková} and Veronika {Faltusová} and Antonio {Pompeiano} and Markéta {Vaculovičová} and Ondřej {Zítka} and Tomáš {Vaculovič} and Vojtěch {Adam}",
  title="Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods",
  journal="International Journal of Biological Macromolecules",
  year="2022",
  volume="203",
  number="1",
  pages="583--592",
  doi="10.1016/j.ijbiomac.2022.01.139",
  issn="0141-8130",
  url="https://doi.org/10.1016/j.ijbiomac.2022.01.139"
}

Documents

1-s2.0-S0141813022001544-main