Development of a Multiplex PCR Assay for Simultaneous Detection of Closely Related Fruit Species in Food

abstract

English

Food adulteration is a long-standing problem. As new ways of adulteration develop, new methods for food authenticity checking, including DNA-based ones, should be developed, too. In this work we focused on multiplex real-time PCR-HRM and its possible use in simultaneous detection of various fruit species in food. New apricot-specific primers were also designed as a part of this work. Peach and apricot were chosen as the fruit species of interest because they occur as both the adulterant and the adulterated commodity [1], and because certain foodstuffs (such as infant food)contain both of these fruits. We analyzed three fruit purees, two of which contained approximately 70 % of apple and 30 % of either apricot or peach. In these purees, neither apricot nor peach was detected. The third puree contained both apricot and peach, but not apple. Using multiplex PCR, we were able to detect apricot in the last puree by both HRM analysis and electrophoresis, and peach was detected by electrophoresis. From these results we conclude that while multiplex PCR can be used for simultaneous detection of peach and apricot in certain foodstuffs, further study focusing on the influence of matrix type on the results of this method wouldbe appropriate.

real-time PCR, multiplex PCR, food authenticity, peach, apricot

FIALOVÁ, L.; LANGOVÁ, D.; PRACHÁROVÁ, A.; MÁROVÁ, I.

25. 11. 2021

ISBN 978-80-214-6002

Studentská odborná konference CHEMIE JE ŽIVOT 2021 Sborník abstraktů

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