Publication result detail

The first insight on changes in PHA granules after extraction from extremophilic microorganisms

MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.

Original Title

The first insight on changes in PHA granules after extraction from extremophilic microorganisms

English Title

The first insight on changes in PHA granules after extraction from extremophilic microorganisms

Type

Abstract

Original Abstract

Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.

English abstract

Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.

Keywords

PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans

Key words in English

PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans

Authors

MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.

RIV year

2022

Released

13.09.2021

Book

Microscopy 2021: Conference

Edition

1

Pages from

81

Pages to

81

Pages count

1

BibTex

@misc{BUT175387,
  author="Kateřina {Mrázová} and Ivana {Nováčková} and Xenie {Kouřilová} and Stanislav {Obruča} and Vladislav {Krzyžánek}",
  title="The first insight on changes in PHA granules after extraction from extremophilic microorganisms",
  booktitle="Microscopy 2021: Conference",
  year="2021",
  series="1",
  edition="1",
  pages="81--81",
  note="Abstract"
}