Pro-healing protein release from 3D-printed „smart“ hydrogel carriers applicable in regenerative medicine

Pro-healing protein release from 3D-printed „smart“ hydrogel carriers applicable in regenerative medicine

Abstract

In recent years, there has been an increase in the use of 3D printing technologies in regenerative medicine to produce medical implants. This work deals with the 3D printing of a hydrogel carrier based on itaconyl-modified PLGA-PEG-PLGA thermosensitive copolymer, which can be crosslinked with blue light in the presence of a water-soluble nontoxic photoinitiator. The copolymer for 3D printing is water soluble, and bioresorbable in the human body. The printing process was performed by direct ink writing method at ambient temperature between 20-40 °C to avoid bioactive substances damage (e.g., growth factors) incorporated into the copolymer water solution before the printing process. A thermostable 9-point mutant of fibroblast growth factor 2 (FGF2-STAB®., Enantis L.t.d.) was chosen as the ideal bioactive component for its ability to support the growth and the formation of new vessels (angiogenesis), leading to improved wound healing, tissue regeneration and contributes to the pathogenesis of several diseases (cancer or atherosclerosis). This work was focused on the FGF2-STAB® protein release monitoring of from a 3D printed hydrogel matrix. The effect of the copolymer degradation at physiological solution on the amount of released protein was monitored by various methods, namely UV-VIS spectrophotometry and SDS-page electrophoresis. The release kinetics and changes between the different modification of used materials were monitored. Based on the results one-step first-order release kinetic was observed. In recent years, there has been an increase in the use of 3D printing technologies in regenerative medicine to produce medical implants. This work deals with the 3D printing of a hydrogel carrier based on itaconyl-modified PLGA-PEG-PLGA thermosensitive copolymer1, which can be crosslinked with blue light in the presence of a water-soluble nontoxic photoinitiator2. The copolymer for 3D printing is water soluble, and bioresorbable in the human body. The printing process was performed by direct ink writing method at ambient temperature between 20-40 °C to avoid bioactive substances damage (e.g., growth factors) incorporated into the copolymer water solution before the printing process. A thermostable 9-point mutant of fibroblast growth factor 2 (FGF2-STAB®., Enantis L.t.d.) was chosen as the ideal bioactive component for its ability to support the growth and the formation of new vessels (angiogenesis), leading to improved wound healing, tissue regeneration and contributes to the pathogenesis of several diseases (cancer or atherosclerosis). This work was focused on the FGF2-STAB® protein release monitoring of from a 3D printed hydrogel matrix. The effect of the copolymer degradation at physiological solution on the amount of released protein was monitored by various methods, namely UV-VIS spectrophotometry and SDS-page electrophoresis. The release kinetics and changes between the different modification of used materials were monitored. Based on the results one-step first-order release kinetic was observed.

In recent years, there has been an increase in the use of 3D printing technologies in regenerative medicine to produce medical implants. This work deals with the 3D printing of a hydrogel carrier based on itaconyl-modified PLGA-PEG-PLGA thermosensitive copolymer, which can be crosslinked with blue light in the presence of a water-soluble nontoxic photoinitiator. The copolymer for 3D printing is water soluble, and bioresorbable in the human body. The printing process was performed by direct ink writing method at ambient temperature between 20-40 °C to avoid bioactive substances damage (e.g., growth factors) incorporated into the copolymer water solution before the printing process. A thermostable 9-point mutant of fibroblast growth factor 2 (FGF2-STAB®., Enantis L.t.d.) was chosen as the ideal bioactive component for its ability to support the growth and the formation of new vessels (angiogenesis), leading to improved wound healing, tissue regeneration and contributes to the pathogenesis of several diseases (cancer or atherosclerosis). This work was focused on the FGF2-STAB® protein release monitoring of from a 3D printed hydrogel matrix. The effect of the copolymer degradation at physiological solution on the amount of released protein was monitored by various methods, namely UV-VIS spectrophotometry and SDS-page electrophoresis. The release kinetics and changes between the different modification of used materials were monitored. Based on the results one-step first-order release kinetic was observed. In recent years, there has been an increase in the use of 3D printing technologies in regenerative medicine to produce medical implants. This work deals with the 3D printing of a hydrogel carrier based on itaconyl-modified PLGA-PEG-PLGA thermosensitive copolymer1, which can be crosslinked with blue light in the presence of a water-soluble nontoxic photoinitiator2. The copolymer for 3D printing is water soluble, and bioresorbable in the human body. The printing process was performed by direct ink writing method at ambient temperature between 20-40 °C to avoid bioactive substances damage (e.g., growth factors) incorporated into the copolymer water solution before the printing process. A thermostable 9-point mutant of fibroblast growth factor 2 (FGF2-STAB®., Enantis L.t.d.) was chosen as the ideal bioactive component for its ability to support the growth and the formation of new vessels (angiogenesis), leading to improved wound healing, tissue regeneration and contributes to the pathogenesis of several diseases (cancer or atherosclerosis). This work was focused on the FGF2-STAB® protein release monitoring of from a 3D printed hydrogel matrix. The effect of the copolymer degradation at physiological solution on the amount of released protein was monitored by various methods, namely UV-VIS spectrophotometry and SDS-page electrophoresis. The release kinetics and changes between the different modification of used materials were monitored. Based on the results one-step first-order release kinetic was observed.

Additive manufacturing, regenerative medicine, tissue engineering, biodegradable hydrogel matrix, protein release

Additive manufacturing, regenerative medicine, tissue engineering, biodegradable hydrogel matrix, protein release

LYSÁKOVÁ, K.; HLINÁKOVÁ, K.; KŘIVÁNKOVÁ, N.; VOJTOVÁ, L.

2022

30.08.2021

Materials Research Society of Serbia

Belgrade

978-86-919111-6-4

THE TWENTY-SECOND ANNUAL CONFERENCE YUCOMAT 2021 Program and Book of Abstracts

82

82

146

https://www.mrs-serbia.org.rs/files/YUCOMAT-2021.pdf

http://hdl.handle.net/