Publication result detail

Environmentally-friendly Isolation of Polyhydroxyalkanoates from Extremophilic Bacteria

NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; MRÁZOVÁ, K.; OBRUČA, S.

Original Title

Environmentally-friendly Isolation of Polyhydroxyalkanoates from Extremophilic Bacteria

English Title

Environmentally-friendly Isolation of Polyhydroxyalkanoates from Extremophilic Bacteria

Type

Abstract

Original Abstract

Biotechnological production of PHA employing extremophiles within concept of Next-Generation Industrial Biotechnology (NGIB) bring many benefits to whole process considering requirement for sterility, cultivation, and also specific possibilities for PHA isolation process. Within our work, we focused on two extremophilic producers, halophilic bacterium Halomonas halophila (CCM 3662) and thermophilic bacterial strain Schlegelella thermodepolymerans (DSM 15344). Unique features of selected extremophiles are used for development of environmentally friendly and also economically feasible isolation approach combining hypotonic shock, elevated temperature and detergent sodium dodecyl sulphate (SDS), which can lead to PHB with purity higher than 90 wt.%. Moreover, we will focuse on recyclation of residual SDS, which represents pollutant of wastewater after isolation.

English abstract

Biotechnological production of PHA employing extremophiles within concept of Next-Generation Industrial Biotechnology (NGIB) bring many benefits to whole process considering requirement for sterility, cultivation, and also specific possibilities for PHA isolation process. Within our work, we focused on two extremophilic producers, halophilic bacterium Halomonas halophila (CCM 3662) and thermophilic bacterial strain Schlegelella thermodepolymerans (DSM 15344). Unique features of selected extremophiles are used for development of environmentally friendly and also economically feasible isolation approach combining hypotonic shock, elevated temperature and detergent sodium dodecyl sulphate (SDS), which can lead to PHB with purity higher than 90 wt.%. Moreover, we will focuse on recyclation of residual SDS, which represents pollutant of wastewater after isolation.

Keywords

PHA, SDS, Halomonas halophila, Schlegelella thermodepolymerans, NGIB

Key words in English

PHA, SDS, Halomonas halophila, Schlegelella thermodepolymerans, NGIB

Authors

NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; MRÁZOVÁ, K.; OBRUČA, S.

RIV year

2022

Released

01.12.2021

ISBN

2564-615X

Periodical

EuroBiotech Journal

Volume

5

Number

2

State

Republic of Italy

Pages from

99

Pages to

99

Pages count

1

URL

https://sciendo.com/issue/EBTJ/5/s2

BibTex

@misc{BUT173031,
  author="Ivana {Nováčková} and Xenie {Kouřilová} and Kateřina {Mrázová} and Stanislav {Obruča}",
  title="Environmentally-friendly Isolation of Polyhydroxyalkanoates from Extremophilic Bacteria",
  year="2021",
  journal="EuroBiotech Journal",
  volume="5",
  number="2",
  pages="99--99",
  url="https://sciendo.com/issue/EBTJ/5/s2",
  note="Abstract"
}