Publication detail

Extending the applicability of in ovo and ex ovo chicken chorioallantoic membrane assays to study cytostatic activity in neuroblastoma cells

MERLOS RODRIGO, M. CASAR, B. MICHÁLKOVÁ, H. JIMENEZ JIMENEZ, A. HEGER, Z. ADAM, V.

Original Title

Extending the applicability of in ovo and ex ovo chicken chorioallantoic membrane assays to study cytostatic activity in neuroblastoma cells

Type

journal article in Web of Science

Language

English

Original Abstract

Purpose The chick chorioallantoic membrane (CAM) assay can provide an alternative versatile, cost-effective, and ethically less controversial in vivo model for reliable screening of drugs. In the presented work, we demonstrate that CAM assay (in ovo and ex ovo) can be simply employed to delineate the effects of cisplatin (CDDP) and ellipticine (Elli) on neuroblastoma (Nbl) cells in terms of their growth and metastatic potential. Methods The Nbl UKF-NB-4 cell line was established from recurrent bone marrow metastases of high-risk Nbl (stage IV, MYCN amplification, 7q21 gain). Ex ovo and in ovo CAM assays were optimized to evaluate the antimetastatic activity of CDDP and Elli. Immunohistochemistry, qRT-PCR, and DNA isolation were performed. Results Ex ovo CAM assay was employed to study whether CDDP and Elli exhibit any inhibitory effects on growth of Nbl xenograft in ex ovo CAM assay. Under the optimal conditions, Elli and CDDP exhibited significant inhibition of the size of the primary tumor. To study the efficiency of CDDP and Elli to inhibit primary Nbl tumor growth, intravasation, and extravasation in the organs, we adapted the in ovo CAM assay protocol. In in ovo CAM assay, both studied compounds (CDDP and Elli) exhibited significant (p < 0.001) inhibitory activity against extravasation to all investigated organs including distal CAM. Conclusions Taken together, CAM assay could be a helpful and highly efficient in vivo approach for high-throughput screening of libraries of compounds with expected anticancer activities.

Keywords

extracranial solid tumor; neuroblastoma; CAM assay; metastasis; preclinical trials; drug testing

Authors

MERLOS RODRIGO, M.; CASAR, B.; MICHÁLKOVÁ, H.; JIMENEZ JIMENEZ, A.; HEGER, Z.; ADAM, V.

Released

1. 9. 2021

Publisher

Frontiers

ISBN

2234-943X

Periodical

Frontiers in Oncology

Year of study

11

Number

9

State

Swiss Confederation

Pages from

1

Pages to

10

Pages count

10

URL

https://www.frontiersin.org/articles/10.3389/fonc.2021.707366/full

Full text in the Digital Library

http://hdl.handle.net/11012/201762

BibTex

@article{BUT172681,
  author="Miguel Ángel {Merlos Rodrigo} and Berta {Casar} and Hana {Michálková} and Ana Maria {Jimenez Jimenez} and Zbyněk {Heger} and Vojtěch {Adam}",
  title="Extending the applicability of in ovo and ex ovo chicken chorioallantoic membrane assays to study cytostatic activity in neuroblastoma cells",
  journal="Frontiers in Oncology",
  year="2021",
  volume="11",
  number="9",
  pages="1--10",
  doi="10.3389/fonc.2021.707366",
  issn="2234-943X",
  url="https://www.frontiersin.org/articles/10.3389/fonc.2021.707366/full"
}